Table of Contents
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FOREWORD
PREFACE
List of Contributors
Bioremediation of Hydrocarbons and Xenobiotic Compound
Abstract
1. INTRODUCTION
2. Types of Bioremediation
3. Types of Hydrocarbons (Aliphatic and Aromatic), Including Xenobiotic Compounds
4. Status and Toxicity of Hydrocarbons and Xenobiotic Compounds
5. Sources of Contaminations
6. Strategies for Eradication/degradation of Xenobiotic Compounds
6.1. Phyto-remediation
6.2. Phyto-degradation
6.3. Phyto-volatilization
6.4. Phyto-extraction
6.5. Phytostabilization
6.6. Rhizoremediation
6.7. Rhizofilteration or Phytofilteration
6.8. Hydraulic Control
6.9. Plant Species Used for Phytoremediation
6.10. Microbe Assisted Phytoremediation of Pollutants
7. Microbial Remediation of Pollutants
7.1. Microbial Degradation of Hydrocarbons in Aerobic Condition
7.1.1. Aerobic Degradation of Aliphatic Hydrocarbons
7.1.2. Aerobic Degradation of Aromatic Hydrocarbons
7.2. Microbial Degradation of Hydrocarbons in Anaerobic Condition
7.2.1. Anaerobic Degradation of Aliphatic Hydrocarbons
7.2.2. Anaerobic Degradation of Aromatic Hydrocarbons
8. CHALLENGES AND RECENT APPROACHES FOR THE MANAGEMENT OF BIOREMEDIATION
8.1. Challenges In Bioremediation
8.1.1. Nature of Chemical Compound
8.1.2. Selection of Microbial Strain
8.1.3. Environmental Conditions
8.2. Recent Approaches For Bioremediation
8.2.1. Application of Enzyme-Based Methods for Bioremediation
8.2.2. Application of Genetically Modified Microorganisms
8.2.3. Application of Surfactant
8.2.4. Microbial Electrochemical System
9. Pros And Cons Of Bioremediation
CONCLUSION AND FUTURE PERSPECTIVE
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Bioremediation of Heavy Metal Contaminated Sites
Abstract
1. INTRODUCTION
2. SOURCES OF HEAVY METALS
3. HEAVY METAL TOXICITY
4. MECHANISMS OF HEAVY METAL BIOREMEDIATION BY MICROORGANISMS
4.1. Bioremediation via Biosorption
4.2. Precipitation
4.3. Adsorption
4.4. Bioaccumulation
4.5. Biostimulation
4.6. Bioaugmentation
4.7. Biosurfactants
4.8. Oxidation-Reduction Reactions
4.9. Metallothioneins (MTs)
4.10. Factors Affecting Biosorption
5. PHYTOREMEDIATION
5.1. Phytovolatilization
5.2. Phytostabilization
5.3. Phytoextraction
5.4. Plant Mechanisms For Metal Detoxification
5.4.1. Phytochelatins (PC)
5.4.2. Plant Metallothioneins (MTs)
5.4.3. Arbuscular Mycorrhizae
5.5. Microbial Assisted Phytoremediation
5.5.1. Siderophores Production
5.5.2. Phosphate Solubilisation
5.5.3. Aminoacyclopropane-1-Carboxylate Deaminase (ACC) And Indole-3-Acetic Acid (IAA) Production
6. EMERGING APPROACHES FOR MICROBIAL AND ENZYMATIC BIOREMEDIATION
6.1. Genetically Modified Organisms (GMO)
6.2. Biosensors
6.3. Nanotechnology
6.4. Metagenomics
6.4.1. Targeted Metagenomics
6.4.2. Whole-Genome Metagenome Sequencing (WMS)
CONCLUSION AND FUTURE PERSPECTIVES
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Bioremediation of Radioactive Contaminants/ Radioactive Metals
Abstract
1. INTRODUCTION
2. SOURCES OF RADIOACTIVE CONTAMINANTS
3. TYPES OF RADIOACTIVE CONTAMINANTS
3.1. Continuous Radioactive Contaminants
3.2. Occasional Radioactive Contaminants
3.3. Accidental Radioactive Contaminants
3.3.1. Low-Level Waste (LLW)
3.3.2. Intermediate-Level Waste (ILW)
3.3.3. High-Level Waste (HLW)
4. REMEDIATION STRATEGIES MITIGATING RADIOACTIVE CONTAMINANTS/RADIOACTIVE METALS
4.1. Physical Remediation
4.2. Chemical Or Abiotic Methods
5. MICROBIAL REMEDIATION
6. Phytoremediation
7. MECHANISM OF BIOREMEDIATION OF RADIOACTIVE CONTAMINANTS
8. RECENT METHODS/DEVELOPMENTS IN BIOREMEDIATION OF RADIOACTIVE CONTAMINANTS
9. CHALLENGES ENCOUNTERED IN BIOREMEDIATION OF RADIOACTIVE CONTAMINANTS
CONCLUSION AND FUTURE PERSPECTIVE
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Microbial Aided Bioremediation and Sources of Biomedical Waste
Abstract
1. INTRODUCTION
2. SOURCES AND TYPES OF BIOMEDICAL WASTES
2.1. Major Sources
2.2. Minor Sources
3. CHARACTERISTIC OF BIOMEDICAL WASTES
3.1. Infectious Waste
3.2. Pathology Waste
3.3. Genotoxic Waste
3.4. Pharmaceutical Waste
3.5. Heavy Metals Waste
3.6. Radioactive Waste
3.7. Chemical Waste
3.8. Sharps
4. REMEDIATION OF BIOMEDICAL WASTE
4.1. Physical And Chemical Methods
4.1.1. Incineration
4.1.2. Autoclaving
4.1.3. Microwave
4.1.4. Shredding
4.1.5. Plasma Pyrolysis
4.1.6. Deep Burial And Inertization
4.1.7. Chemical-Based Technology
4.1.8. Landfilling
5. MICROBIAL METHODS
6. CHALLENGES IN BIOREMEDIATION OF BIOMEDICAL WASTES
7. RECENT DEVELOPMENTS IN THE BIOREMEDIATION OF BIOMEDICAL WASTES
CONCLUSION AND PERSPECTIVE
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Electronic Waste Management: An Emerging Challenge to the Environment
Abstract
1. INTRODUCTION
2. IMPACT OF E-WASTE ON ECOSYSTEM
3. RECYCLING AND MANAGEMENT OF E-WASTE
3.1. Pyrometallurgical Process
3.2. Thermochemical Method
3.3. Hydrometallurgical Method
4. BIOLEACHING OF METALS FROM E-WASTE
5. PERSPECTIVES FOR E-WASTE MANAGEMENT BY MICROORGANISMS
6. CHALLENGES AND RECOMMENDATIONS FOR E-WASTE MANAGEMENT
CONCLUSION AND FUTURE PERSPECTIVES
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOLEDGEMENTS
REFERENCES
Bioremediation Strategies for the Decontamination of Solid Waste Pollutants
Abstract
1. INTRODUCTION
2. TOXIC POLLUTANTS CONTAMINATED FROM SOLID WASTE IN THE ENVIRONMENT
2.1. Heavy Metal From Industrial Sludge
2.2. Heavy Metal From Municipal Sludge
2.3. Ecological Toxicity Of Heavy Metal
3. REMEDIATION PROCESS AVAILABLE FOR SOLID WASTE SLUDGE
3.1. Phytoremediation Of Solid Waste Sludge
4. ROLE OF METAL TOLERANT BACTERIA IN BIOREMEDIATION OF SLUDGE
5. ROLE OF ARBUSCULAR MYCORRHIZAE FUNGI (AMF) IN HEAVY METAL REMOVAL
6. MECHANISTIC BEHAVIOR OF PLANT-MICROBE INTERACTION FOR METAL REMOVAL
6.1. Mechanism Of Phytoextraction
6.2. Phytovolatilization Of Metal Ions
6.3. Phytostabilization Of Toxic Metal
6.4. Rhizofiltration: An Efficient Process
6.5. Role Of Root Exudates In Metal Chelation
6.6. Restriction Of Metal Uptake Through The Plasma Membrane
6.7. Role Of Phytochelatins In Metal Detoxification
CONCLUSION
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Application of “OMICs” Approaches in Bioremediation
Abstract
1. INTRODUCTION
2. GENOMICS IN BIOREMEDIATION
2.1. Genomics- To Know What Is There
2.2. Genomics- Implemented For Bioremediation
3. TRANSCRIPTOMICS IN BIOREMEDIATION
3.1. Transcriptomics Implemented For Bioremediation
4. PROTEOMICS
4.1. Metaproteomics
4.1.1. Application Of Metaproteomics In Soil Bioremediation
4.1.2. Applications Of Metaproteomics In Marine And Freshwater Bioremediation
5. KEY CANDIDATES USED IN BACTERIAL BIOREMEDIATION
6. METABOLOMICS
6.1. Profiling (DIFFERENTIAL EXPRESSION ANALYSIS)
6.1.1. Compound Identification
6.1.2. Interpretation
6.2. Metabolomics In Bioremediation
7. MICROBIAL SPECIES USED IN PESTICIDE BIOREMEDIATION
CONCLUSION AND FUTURE PERSPECTIVE
CONSENT FOR PUBLICATION
CONFLICT OF INTEREST
ACKNOWLEDGEMENTS
REFERENCES
Management of Agriculture Waste: Bioconversion of Agro-Waste into Valued Products
Abstract
1. INTRODUCTION
2. PRODUCTION OF VALUE-ADDED PRODUCTS
2.1. Enzymes
2.1.1. Production Of Polysaccharide Hydrolyzing Enzymes
2.1.2. Production Of Protein Hydrolyzing Enzymes
2.1.3. Production Of Other Industrially Valued Enzymes
2.2. Ethanol
2.2.1. Pretreatment Of Lignocellulose
2.2.2. Saccharification
2.2.3. Fermentation
2.3. Biogas
2.4. Compost
2.4.1. Vermicompost
Method Of Vermicompost Production
2.5. Mushrooms
2.6. Animal Feeds
2.6.1. Limitations Of Agro-Wastes As Animal Feed
2.6.2. Methods For The Development Of Animal Feed From Agro-Wastes
3. CHALLENGES AND ADVANCEMENTS
CONCLUSION AND FUTURE PERSPECTIVES
CONSENT FOR PUBLICATION
CONFLICTS OF INTEREST
ACKNOWLEDGMENTS
Bioremediation: Challenges and Advancements
Edited by
Manikant Tripathi
Biotechnology Programme
Dr. Rammanohar Lohia Avadh University,
Ayodhya-224001
Uttar Pradesh
India
&
Durgesh Narain Singh
Department of Zoology
University of Delhi
Delhi-110007
India, and BioNEST-BHU
Banaras Hindu University
Varanasi-221005, U.P.
India
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FOREWORD
Prof. Rup Lal
NASI Senior Scientist Platinum Jubilee Fellow,
The Energy and Resources Institute (TERI), Lodhi Road, New Delhi-110003, India.
(Former Professor, Department of Zoology, University of Delhi, Delhi-110007)
[email protected]The process of production and consumption to meet the incessant demands of mankind leads to the generation of different undesirable pollutants and waste materials. Such undesirable products are potential threats to the environment and pose different kinds of risks to mankind. We need a proper management system and decontamination technologies for the abatement of pollutants and waste materials in a sustainable manner. Most of the decontamination methods are not only costly and energy-consuming, but the generated byproducts are toxic to the environment. Nevertheless, bioremediation is an ecofriendly and economical method that employs different types of microorganisms for the removal of pollutants from the environment. Microorganisms utilize several strategies to remove contaminants, including enzymatic detoxification, adsorption to cell surfaces, intracellular accumulation, sequestration into exopolysaccharides, volatilizations, and biotransformation into their non-toxic form. The wide metabolic and physiological capabilities of microorganisms allow them to survive in extreme environments. These properties render microorganisms the incredible potential of bioremediation, but microorganisms also meet several challenges when applied to the environment for bioremediation. Researchers are working consistently to combat challenges for the successful development of new bioremediation technologies.
This book titled “Bioremediation: Challenges and Advancements” discusses the concepts of bioremediation, challenges, and advancement in bioremediation of different pollutants such as hydrocarbons, xenobiotics, heavy metals, radioactive compounds, and phytoremediation of industrial wastes. Some of the chapters that make the book unique and distinguish it from its contemporaries are the management of plastic wastes and e-wastes, biomedical wastes, and the management of agricultural wastes. Another very interesting chapter is “Application of ‘omics’ in bioremediation.” I hope this book will be beneficial for undergraduate and postgraduate students, researchers, and environmental scientists involved in the bioremediation of different contaminated sites.
Prof. Rup Lal
The Energy and Resources Institute (TERI), Lodhi Road
New Delhi-110003, India
(Former Professor, Department of Zoology, University of
Delhi, Delhi-110007).
India
PREFACE
DURGESH NARAIN SINGH MANIKANT TRIPATHIWaste management is one of the major challenges for environmental and public health worldwide. With the growing population and urbanization, it is increasing every day. These wastes contain various types of toxic pollutants of organic and inorganic nature, which disturb the ecosystem and pose adverse effects to humans and animals. The provision of pollution-free soil, air, and safe water is critical for a balanced ecosystem. Several treatment techniques, including physical, chemical, and biological, are used to remediate hazardous wastes, whereas bioremediation offers cost-effective and green technology for the abatement of toxic pollutants in the environment. However, there are lots of constraints and complexity in dealing with waste management. This book comprises eight chapters that focus on the waste source to its adverse impacts on the ecosystem, and the advanced strategies for their remediation along with associated challenges. The first two chapters explain microbe-assisted remediation technology to detoxify heavy metals and degrade xenobiotic compounds through various biosynthetic mechanisms, along with the significance of recent biotechnological methods in improving the capability of microbial remediation methods. The toxic pollutants are successfully degraded in microbe-assisted remediation along with phytoremediation. The challenges, future outlooks, and limitations are also discussed. Chapter 3 describes strategies including physical, chemical, and biological methods to mitigate radioactive waste from contaminated sites and water bodies. This chapter is focused on eco-friendly and economical solutions for global radioactive waste disposal problems and other associated challenges. Chapter 4 discusses biomedical waste, which is hazardous, and if left untreated, can cause serious health hazards. This chapter detailed the use of microbial-aided remediation techniques for the removal of biomedical waste. Chapter 5 explains the source of electronic waste, its impact on humans and animals, physicochemical and advanced microbiological methods for the management of e-waste, as well as challenges associated with this. Chapter 6 describes bioremediation strategies for the decontamination of solid waste pollutants. Chapter 7 focuses on the application of OMICs approaches such as genomics, transcriptomics, proteomics, and metabolomics in bioremediation. It plays a significant role in generating information about degradative enzymes and pathways involved in the remediation of pollutants by microorganisms. The last chapter describes the bioremediation of agricultural wastes.
The peculiarity of the book is that it does not only cover methods of bioremediation but also describes the challenges as well recent advancements in the bioremediation of different pollutants. This book would be beneficial to students of environmental sciences, including microbiology and biotechnology, environmental engineers, and researchers working for the restoration of contaminated sites.
Manikant Tripathi
Biotechnology Programme
Dr. Rammanohar Lohia Avadh University
Ayodhya-224001
Uttar Pradesh
India
&Durgesh Narain Singh
Department of Zoology
University of Delhi, Delhi-110007
India
and BioNEST-BHU, Banaras Hindu
List of Contributors
Singh Durgesh Narain, Department of ZoologyUniversity of Delhi, DelhiUniversity of Delhi, DelhiBioNEST-BHUBanaras Hindu UniversityVaranasi-221005U.P., IndiaSingh Geetu, Division of Agronomyand Soil ScienceCSIR-Central Institute of Medicinal and Aromatic PlantsLucknowIndiaKhurana Himani, Gut Biology Laboratory, Roomno-117 Department of ZoologyUniversity of DelhiDelhiIndiaGautam Krishna, Academy of Scientific and Innovative Research (AcSIR)CSIR-Indian Institute of Toxicology ResearchLucknow, Uttar PradeshIndiaTripathi Manikant, Biotechnology ProgrammeDr. Rammanohar Lohia Avadh UniversityAyodhya, Uttar PradeshIndiaSharma Monika, Fish Molecular Biology LaboratoryDepartment of Zoology, University of DelhiDelhiIndiaPathak Neelam, Department of BiochemistryDr. Rammanohar Lohia Avadh UniversityAyodhya, Uttar PradeshIndiaYadav Neetu Singh, Department of BiotechnologyDeen Dayal Upadhyaya Gorakhpur UniversityGorakhpur, Uttar PradeshIndiaPrasad Nivedita, Department of Dairy Microbiology, Sam Higginbottom University of AgricultureTechnology and Sciences (SHUATS)PrayagrajIndiaSharma Poonam, Department of BioengineeringIntegral UniversityLucknow, Uttar PradeshIndiaGaur Prachi, Indian Institute of Manangement and Technology College AligarhUttar PradeshIndiaRahul Rahul, Fish Molecular Biology LaboratoryDepartment of Zoology, University of DelhiNew DelhiIndiaGaur Rajeeva, Department of MicrobiologyDr. Rammanohar Lohia Avadh UniversityAyodhya, Uttar PradeshIndiaVerma Rajesh Kumar, College of Horticulture and ForestryRani Lakshmi Bai Central Agricultural UniversityJhansi, Uttar PradeshIndiaSingh Rakshpal, CSIR-Central Institute of Medicinal and Aromatic Plants LucknowUttar PradesIndiaNegi Ram Krishan, Fish Moleculr Biology LaboratoryDepartment of Zoology,University of DelhiDelhiIndiaKushwaha Ramesh Kumar, Department of BiochemistryREVA UniversityBangaloreIndiaJamwal Rohit, Soil Microbial Ecology and Environmental Toxicology Laboratory Department of ZoologyUniversity of DelhiDelhiIndiaSrivastava Sanjeev, Department of Environmental SciencesDr.Rammanohar Lohia Avadh UniversityAyodhya, Uttar PradeshIndiaSharma Shagun, Immunobiology Laboratory, Department of ZoologUniversity of DelhiDelhiIndiaMazumder Shibnath, Immunobiology Laboratory, Department of ZoologUniversity of DelhiDelhiIndiaSingh Sucheta, Molecular Biology and Biotechnology DivisionCSIR-National Botanical Research InstituteLucknowIndiaSingh Sudhir, Microbiology and Cell BiologyBiological Science Indian Institute of ScienceBangaloreKarnataka IndiaSingh Suman, Department of BotanyDepartment of BotanyLucknowIndiaPankaj Umesh, College of Horticulture and ForestryRani Lakshmi Bai Central Agricultural UniversitJhansi, Uttar PradeshIndiaShankar Singh Vijay, School of BiotechnologySchool of BiotechnologyVaranasiIndiaGaur Vivek Kumar, Amity Institute of BiotechnologyAmity University Uttar Pradesh, Lucknow CampusLucknow, Uttar PradeshIndia
Bioremediation of Hydrocarbons and Xenobiotic Compound
Suman Singh1,Sucheta Singh2,Ramesh Kumar Kushwaha3,*
1 Department of Botany, University of Lucknow-226007, India
2 Molecular Biology and Biotechnology Division, CSIR-National Botanical Research Institute, Lucknow-226001, India
3 Department of Biochemistry, REVA University, Bangalore-560064, India
Abstract
In the last few decades, the increase in population, the industrial revolution, and modernization have produced numerous problems in the form of hazardous pollutants in the ecosystem rapidly. These hazardous pollutants such as polycyclic aromatic hydrocarbons (PAHs), heavy metals, manmade pesticides (xenobiotics), radioactive materials, toxic chemicals, and dyes created an imbalance in the ecosystem and increased risks to human, plants, and animal’s health. Furthermore, the use of chemical fertilisers, pesticides, and sewage releases toxicants into the soil and potable water, where they enter the food chain and endanger food security. Many strategies and practices have been used to prevent harmful effects of these pollutants up to a certain extent. Various physical and chemical methods have been implemented to remove these contaminants, but due to some limitations, it has not been applied successfully. Despite this, appropriate biological methods are currently applied to decrease pollutants’ concentrations from the soil, water, and the environment. The use of biological methods for bioremediation should be cost-effective, eco-friendly, and biodegradable, decreasing the danger to the ecosystem and living beings. Microbe-assisted remediation technology has been developed to degrade xenobiotic compounds through various biosynthetic mechanisms. The objective of this chapter is to discuss different methods of bioremediation, their process, and mechanisms, employing potential plants and microbes in the remediation of pollutants from the environment. In addition, the present chapter highlighted the significance of recent biotechnological methods in improving the capability of microbial remediation methods. These methods successfully degrade pollutants, emphasizing current advances in microbe-assisted remediation along with phytoremediation as well as related challenges, future outlooks, and limitations.
Keywords: Bioremediation, Chemical Fertilizers, Ecosystem, Heavy Metals, Microbial Remediation, Pesticides.
*Corresponding author Ramesh Kumar Kushwaha: Department of Biochemistry, REVA University, Bangalore-560064, India; Tel:+918004425060; E-mails:
[email protected],
[email protected]