How Stimulus-responsive Extracellular Vesicle Release is Regulated and Associated to Lewy body disease - Rohit Kumar - E-Book

How Stimulus-responsive Extracellular Vesicle Release is Regulated and Associated to Lewy body disease E-Book

Rohit Kumar

0,0
39,99 €

-100%
Sammeln Sie Punkte in unserem Gutscheinprogramm und kaufen Sie E-Books und Hörbücher mit bis zu 100% Rabatt.
Mehr erfahren.
  • Herausgeber: GRIN Verlag
  • Sprache: Englisch
  • Veröffentlichungsjahr: 2023
Beschreibung

Doctoral Thesis / Dissertation from the year 2021 in the subject Biology - Neurobiology, grade: N/A, Technical University of Munich (German Center for Neurodegenerative Diseases (DZNE)), language: English, abstract: Lewy body (LB) disorders, such as dementia with LBs and Parkinson’s disease with dementia (PDD) are characterized by their pathological hallmark, i.e., the gradual appearance of LBs. Therefore, a deep understanding of the molecular and cellular mechanism, favoring the formation of LBs is required.These proteinaceous inclusions are found in different cell types of the nervous system. The key constituent protein-component of such inclusions is the protein alpha-synuclein (α-Syn). α-Syn along with other bio-active material gets secreted as content of extracellular vesicles (EVs). These vesicles not only excrete bioactive material but are also able to transport it to distant sites, and therefore have implications for disease pathology seeding and spreading.Using patch-clamp electrophysiology techniques along with simultaneous real-time molecular imaging, we determined the kinetics of EV-release in hippocampal neurons. We designed a pH-sensitive molecular tag, targeted specifically to membranes of EVs and applied this method to study stimulus-responsive MVB-PM fusion, in order to determine the kinetics, specific stimuli and regulators of such events. In addition, we analyzed the EV-content by performing mass spectrometry (MS) analysis and identifyed LBP-related molecular interactions.

Das E-Book können Sie in einer beliebigen App lesen, die das folgende Format unterstützt:

PDF
Bewertungen
0,0
0
0
0
0
0
Mehr Informationen
Mehr Informationen
Legimi prüft nicht, ob Rezensionen von Nutzern stammen, die den betreffenden Titel tatsächlich gekauft oder gelesen/gehört haben. Wir entfernen aber gefälschte Rezensionen.