Synthetic Biology E-Book

• Herausgeber: Wiley-VCH
• Kategorie: Wissenschaft und neue Technologien
• Serie: Current Topics from the Encyclopedia of Molecular Cell Biology and Molecular Medicine
• Sprache: Englisch

Seitenzahl: 1682

Veröffentlichungsjahr: 2015

Beschreibung

These two volumes contain a selection of updated articles from the acclaimed Meyers Encyclopedia of Molecular Cell Biology and Molecular Medicine, the most authoritative resource in cell and molecular biology, combined with new articles by "founding fathers" in the field. The work is divided into six sections: + Biological Basis + Modeling + Modular Parts and Circuits + Synthetic Genomes + Diseases and Therapeutics + Chemicals Production. Ideally suited as advanced reading for students and postdocs, and with all current research trends covered by an impressive number of leading figures in the field, this is the first choice reference for research institutions.

Leseprobe

Table of Contents

Cover

Related Titles

Title Page

Copyright

Preface

Volume 1

Chapter 1: Synthetic Biology: Implications and Uses

1 Introduction

2 DNA Assembly and Modification

3 Modular Parts and Circuits

4 Spatial Regulation

5 The Synthetic Cell

6 Societal Challenges Posed by Synthetic Biology

7 Concluding Remarks

Acknowledgments

References

Part I: Biological Basis

Chapter 2: The Emergence of the First Cells

1 Introduction

2 Origin of Prebiotic Metabolism and Compartments

3 Origin of Extant Cells

4 Conclusion

References

Chapter 3: Regulation of Gene Expression

1 Introduction

2 Regulation of Gene Expression in Prokaryotes

3 Regulation of Gene Expression in Eukaryotes

4 RNA Splicing

5 Role of microRNAs (miRNAs) in the Regulation of Gene Expression

6 Chromatin Structure and the Control of Gene Expression

7 Epigenetic Control of Gene Expression

8 Gene Regulation by Hormonal Action

9 Post-Transcriptional Regulation of mRNA

10 Transport of Processed mRNA to the Cytoplasm

11 Regulation of Gene Expression at the Level of Translation

Acknowledgments

References

Chapter 4: Interactome

1 Introduction

2 Experimental Techniques for Detecting Protein Interactions

3 Computational Prediction of Protein Interactions

4 Exploring the Topology of the Interactome

5 Comparing Protein–Protein Interaction Networks

6 Databases of Protein and Domain Interactions

7 Applications

8 Looking Ahead: Towards the Dynamic Interactome

Acknowledgments

References

Chapter 5: Microbiomes

1 Introduction

2 History of Microbial Diversity Studies

3 Microbiomes

4 Single-Cell Genomics

5 Sequence Technologies and Tools

6 Future Perspectives

Acknowledgments

References

Part II: Modeling

Chapter 6: Dynamics of Biomolecular Networks

1 Introduction

2 Boolean Dynamics Models

3 Continuous Dynamics Models

References

Chapter 7: E-Cell: Computer Simulation of the Cell

1 Introduction

2 Biological Modeling and Simulation Tools

3 The E-Cell System

4 Practical Applications

5 Concluding Remarks

References

Chapter 8: SynBioSS Designer Modeling Suite

1 Introduction

2 Modeling Synthetic Systems Made by BioBricks

3 Modeling Synthetic Systems Made by User-Defined Genetic Constructs

4 Current Limitations of the Designer

5 Conclusions

Acknowledgments

References

Part III: Modular Parts and Circuits

Chapter 9: Synthetic Gene Circuits

1 Introduction to Synthetic Gene Circuits

2 Building Blocks of Synthetic Gene Circuits

3 Dynamical Circuits

4 Memory Devices

5 Boolean Logic and Digital Circuits

6 Analog Circuits

7 Intercellular Communication and Synthetic Multicellular Devices

8 Synthetic Circuit Construction: Challenges and Solutions

9 Applications of Synthetic Circuits

10 Conclusions

Note Added in Proof

References

Chapter 10: DNA Origami Nanorobots

1 Introduction to Robotics

2 DNA Nanotechnology

3 DNA Nanorobotics

4 Challenges of Applying DNA Nanorobots to Therapeutics

5 Summary and Conclusions

References

Chapter 11: RNAi Synthetic Logic Circuits for Sensing, Information Processing, and Actuation

1 Introduction

2 Overview of RNA Interference

3 Applications

4 Conclusions and Perspectives

References

Chapter 12: Synthetic Hybrid Biosensors

1 Introduction

2 Sensor Design

3 Electrochemical Biosensors

4 Optical Biosensors

5 Piezoelectric Biosensors

6 Thermal Biosensors

7 Microarrays

8 Conclusions

Acknowledgments

References

Chapter 13: Synthetic Biology in Metabolic Engineering: From Complex Biochemical Pathways to Compartmentalized Metabolic Processes – a Vitamin Connection

1 Industrial Production of Vitamin B

12

2 Modular Assembly of Cobalamin

3 Nature Has Evolved Different Ways to Catalyze Ring Contraction

4 The Methylases in the Cobalamin Biosynthetic Pathway Are Largely Derived From One Common Ancestor

5 CobA: Enhancing Pathway Productivity through Protein Engineering

6 Channeling Efficiency

7 Biosynthesis of Mismethylated Analogs of Cobalamin

8 Substitution of the Metal Ion

9 Tailoring of the Nucleotide Loop

10 Cobalamin Conjugates

11 Physical Compartments for Pathway Sequestration

12 Future Directions

References

Volume 2

Part IV: Synthetic Genomes

Chapter 14: The Minimal Gene-Set Machinery

1 Introduction

2 Top-Down Approaches to the Minimal Gene Set

3 The Minimal Gene-Set Machinery

4 Conclusions

Acknowledgments

References

Chapter 15: Production of Mitochondrial Genome and Chromosomal DNA Segments Highly Engineered for Use in Mouse Genetics by a Bacillus subtilis-Based BGM Vector System

1 Introduction

2 Giant DNA Handled by the BGM System

3 Production of a Full-Length Mouse Mitochondrial Genome by the BGM System

4 Production of Heavily Manipulated DNA for Mouse Genetics

5 Future Perspectives for the BGM System in Molecular Cloning and Genome Design

References

Chapter 16: Synthetic Genetic Polymers Functioning to Store and Propagate Information by Genetic Alphabet Expansion

1 Introduction

2 Unnatural Base Pairs

3 Applications

4 Conclusion

References

Part V: Diseases and Therapeutics

Chapter 17: Synthetic Biology Approaches for Regenerative Medicine

1 Introduction: Current Problems in Regenerative Medicine

2 Synthetic Biology as a Promising Source of Solutions

3 Ethical, Legal, and Social Implications

References

Chapter 18: The Synthetic Biology Approach to Stem Cells and Regenerative Medicine

1 Introduction

2 Synthetic Gene Circuits for Directing Stem Cell Differentiation and Establishing Intercellular Communication Networks within 3D Tissue-Engineered Constructs

3 Precise Gene Targeting and Genome-Editing Technologies to Allay Safety Concerns of Synthetic Gene Circuits Encoded by Recombinant DNA

4 Non-integrating Vectors for the Expression of Transgenes: Episomal Plasmids and Sendai Virus

5 Modulating Gene Expression through Direct Delivery of Proteins and RNA into the Cell: A Safer Alternative for Stem Cell Differentiation and Reprogramming Compared with Recombinant DNA Transfection

6 Breakthrough: Chemical-Based Reprogramming to Pluripotent Stem Cells with Small Molecules Alone

7 Overcoming the Limited Plasticity and Proliferative Potential of Adult Stem Cells Using a Synthetic Biology Toolkit

8 Conclusions: Safety Issues and Future Outlook

References

Chapter 19: Synthetic Biology Approaches to Cell Therapy

1 Introduction

2 Cell Therapy Successes

3 Synthetic Circuits

4 Cell Therapies Enabled by Synthetic Biology

5 Safety

6 Concluding Remarks

Acknowledgments

References

Chapter 20: Synthetic Biology Approaches for Vaccine Development

1 Introduction

2 Synthetic Approaches to Vaccine Design

3 Attenuation by CPB Recoding

4 Mechanisms of Attenuation by Recoding

5 Eradication as the Goal of Vaccines

6 Concluding Remarks

References

Part VI: Chemicals Production

Chapter 21: Metabolic Engineering for the Production of Diols

1 Introduction

2 1,2-Propanediol (1,2-PD)

3 1,3-Propanediol

4 2,3-Butanediol

References

Chapter 22: Synthetic Biology in Biofuels Production

1 Introduction

2 Synthetic Biology: The Tools and Applications

3 Role of Synthetic Biology in Feedstock Improvement

4 Enzyme Engineering for Hydrolysis of Biomass

5 Microbial Engineering to Expand the Substrate Range

6 Microbial Engineering to Produce Various Biofuel Molecules

7 Role of Synthetic Biology in Algal Fuels

References

Chapter 23: Synthetic Biology of Antibiotic Production

1 Introduction

2 The Need for Chemical Diversity

3 Classical Approaches to Antibiotic Biosynthesis

4 Synthetic Biology Methods for Genome Prospecting of New Bioactive Molecules

5 Synthetic Biology Approaches for the Rapid Generation of Chemical Diversity

6 Engineering of Versatile Chassis for the Synthetic Biology of Antibiotics

7 Tools for the Future

References

Index

End User License Agreement

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