Essential Laboratory Skills for Biosciences E-Book

Mohammed Meah

• Herausgeber: John Wiley & Sons
• Kategorie: Wissenschaft und neue Technologien
• Sprache: Englisch

Seitenzahl: 146

Veröffentlichungsjahr: 2012

Beschreibung

Essential Laboratory Skills for Biosciences is an essential companion during laboratory sessions. It is designed to be simple and give clear step by step instructions on essential techniques, supported by relevant diagrams. The book includes the use of particular equipment and how to do simple calculations that students come across regularly in laboratory practicals.

Written by experienced lecturers this handy pocket book provides:

• Simple to follow laboratory techniques
• Clear use of diagrams and illustrations to explain techniques, procedures and equipment
• Step by step worked out examples of calculations including concentrations, dilutions and molarity

Suitable for all first year university students, the techniques in the book will also be useful for postgraduate and final year project students and enhance the practical and theoretical knowledge of all those studying bioscience related subjects.

Leseprobe

Table of Contents

Title Page

Copyright

List of Figures

List of Tables

Acknowledgements

Introduction

Health and Safety

Chapter 1: Measurements and Calculations

1.1 Units and measurements

1.2 Measuring the volumes of liquids

1.3 Pipetting

1.4 Weighing

1.5 Calculations

Chapter 2: Preparing Solutions

2.1 Common terms defining solutions

2.2 Precautions in making solutions

2.3 Making solutions

2.4 Dilutions to prepare standard solutions

2.5 Molar solutions

2.6 Calculations involving solutions

Chapter 3: Separation of Liquids and Solids

3.1 Filtration

3.2 Centrifugation

3.3 Chromatography

3.4 Electrophoresis

Chapter 4: Common Techniques and Equipment

4.1 Titration

4.2 Spectrophotometry

4.3 Aseptic techniques

4.4 Disinfectants

Chapter 5: Microscopy and Histology

5.1 Light microscopy

5.2 Slide preparation

5.3 Cell Counting

Chapter 6: Cardiorespiratory Measurements

6.1 Techniques to investigate cardiovascular function

6.2 Techniques to investigate respiratory function

Chapter 7: Recording and Presenting Data

7.1 Keeping a laboratory book

7.2 Presentation of data

7.3 Recording data in tables

7.4 Presenting data in graphs

7.5 Describing data statistically

Recommended Reading

Appendix 1: Rules for Powers

Appendix 2: Rules for Logarithms

Appendix 3: Factors to Consider When Making Solutions

Appendix 4: Principle of Spectrophotometry

Appendix 5: Descriptive Statistics and Formulae

Appendix 6: Using Software to Draw Tables, Graphs and Calculating Descriptive Statistics

Index

This edition first published 2012 © 2012 by John Wiley & Sons, Ltd.

Wiley-Blackwell is an imprint of John Wiley & Sons, formed by the merger of Wiley's global Scientific, Technical and Medical business with Blackwell Publishing.

Registered office: John Wiley & Sons, Ltd, The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK

Editorial offices: 9600 Garsington Road, Oxford, OX4 2DQ, UK

The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, UK

111 River Street, Hoboken, NJ 07030-5774, USA

For details of our global editorial offices, for customer services and for information about how to apply for permission to reuse the copyright material in this book please see our website at www.wiley.com/wiley-blackwell

The right of the author to be identified as the author of this work has been asserted in accordance with the UK Copyright, Designs and Patents Act 1988.

All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, except as permitted by the UK Copyright, Designs and Patents Act 1988, without the prior permission of the publisher.

Designations used by companies to distinguish their products are often claimed as trademarks. All brand names and product names used in this book are trade names, service marks, trademarks or registered trademarks of their respective owners. The publisher is not associated with any product or vendor mentioned in this book. This publication is designed to provide accurate and authoritative information in regard to the subject matter covered. It is sold on the understanding that the publisher is not engaged in rendering professional services. If professional advice or other expert assistance is required, the services of a competent professional should be sought.

The contents of this work are intended to further general scientific research, understanding, and discussion only and are not intended and should not be relied upon as recommending or promoting a specific method, diagnosis, or treatment by physicians for any particular patient. The publisher and the author make no representations or warranties with respect to the accuracy or completeness of the contents of this work and specifically disclaim all warranties, including without limitation any implied warranties of fitness for a particular purpose. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of medicines, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each medicine, equipment, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. Readers should consult with a specialist where appropriate. The fact that an organization or Website is referred to in this work as a citation and/or a potential source of further information does not mean that the author or the publisher endorses the information the organization or Website may provide or recommendations it may make. Further, readers should be aware that Internet Websites listed in this work may have changed or disappeared between when this work was written and when it is read. No warranty may be created or extended by any promotional statements for this work. Neither the publisher nor the author shall be liable for any damages arising herefrom.

Library of Congress Cataloging-in-Publication Data

Meah, Mohammed.

Essential laboratory skills for biosciences / Mohammed Meah and Elizabeth Kebede-Weshead.

p. cm.

Includes bibliographical references and index.

ISBN 978-0-470-68647-8 (pbk.)

1. Chemistry–Laboratory manuals. 2. Chemical apparatus–Handbooks, manuals, etc.

I. Kebede-Weshead, Elizabeth. II. Title.

QD45.M39 2011

507.2′1—dc23

2011043893

A catalogue record for this book is available from the British Library.

Wiley also publishes its books in a variety of electronic formats. Some content that appears in print may not be available in electronic books.

List of Figures

Chapter 1Measurements and Calculations

Figure 1.1 Accuracy and precision shown on a target

Figure 1.2a Beaker

Figure 1.2b Conical (Erlenmeyer) flask

Figure 1.2c Measuring cylinder

Figure 1.2d Burette

Figure 1.2e Volumetric flask

Figure 1.2f Pipette

Figure 1.3 Location of the meniscus of a liquid

Figure 1.4 Parts of a Gilson pipette

Figure 1.5 Circle of pipettes

Figure 1.6 Tips used with pipettes

Figure 1.7 Setting the volume on a Gilson pipette

Figure 1.8 Types of pipette tips

Figure 1.9 Pipette inserted into beaker

Figure 1.10 Ejecting tip using tip ejector button

Figure 1.11 Multichannel pipette

Figure 1.12 Pasteur pipette

Figure 1.13 Portable digital top-loading balance

Figure 1.14 Plastic boat

Figure 1.15 Spatula

Figure 1.16 A typical analytical balance

Chapter 2Preparing Solutions

Figure 2.1 Wash bottle

Figure 2.2 Serial dilution of a stock solution