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- Herausgeber: BookRix
- Kategorie: Wissenschaft und neue Technologien
- Sprache: Englisch
This book is useful for pharmacy,Nursing and medical proffession.It contains practical related to urine and blood component. I have expereience in biochemistry field more than 5 years.
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Veröffentlichungsjahr: 2015
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BIOCHEMISTRY OF QUANTITATIVE ESTIMATIONS OF URINE & BLOOD CONSTITUENTS
BookRix GmbH & Co. KG81371 MunichINDEX
INDEX
QUANTITATIVE ESTIMATIONS OF URINE & BLOOD CONSTITUENTS
Sr.No.
Aim of experiment
Page.No.
1.
Introduction to the Laboratory, subject.
2
2.
Estimation of Chlorides in Urine (Whitehorn's Method).
6
3.
To determine titrable acidity and ammonia in urine.
9
4.
To determine the glucose content in urine by Benedict’s method.
12
5.
To determine the glucose content in urine by Cole’s ferricyanide – Methylene blue method.
15
6.
Estimation of serum calcium.
18
7.
Introduction to colorimetric analysis.
21
8.
To estimate total proteins in plasma by biuret method.
23
9.
To estimate creatinine in blood.
26
10.
To estimate total cholesterol in plasma.
29
11.
References
32
INRIDUCTION TO THE LABORATORY
Experiment No: 1
Aim: Introduction to the Laboratory, subject, list of requirement.
Introduction to the Laboratory:
Laboratory work has special importance in the learning of science as scientific principles develop and grow on the basis of experimental work. Chemistry is an experimental science.
Laboratory work provides an opportunity to observe many of the chemical phenomena under controlled laboratory conditions. Hence, it provides you with ample opportunity to observer and to draw inferences and explain results.
Before you start working in the laboratory, you should be well acquainted with the biochemistry laboratory. You should notice the facilities provided in the laboratory and on your working table.
You will notice that your working table is provided with a sink, water-tap, gas-tap, Bunsen burner with guard cum stand, a reagent shelf and a bin for disposing waste material. You will find that some reagents are kept on the shelf fixed on the table while some reagents are kept on the shelf fixed on sidewalls. Reagents kept on the shelf of the table are frequently required while those on the shelf fixed on walls are less commonly used.
You will see that there are exhaust fans fitted on the upper portion of the wall to facilitate the exhaustion of harmful fumes and circulation of fresh air in the laboratory.
It is strongly advised that you should become fully familiar with your biochemistry laboratory, laboratory practices and procedures, and more importantly the precautions to be taken while working in the laboratory.
The chemistry laboratory may become a place of danger if you do not follow the precautions properly in conducting the experiments where potential danger exists. Hence you must learn to handle the equipment and chemicals properly as well as familiarize yourself with the safety measures and good laboratory practices.
You should work individually unless the experiments require teamwork.
Prepare reports of the experiments in the laboratory notebook. Do not use loose sheets or scraped papers for this purpose.
Use books in case of any doubt because books are more reliable, complete and better source of information than classmates. Else consult your teacher.
Safety rules are designed to ensure that the work done in the laboratory is safe for you and your fellow students. Follow the safety rules and be aware of where the items like first-aid box, fire extinguisher etc., are kept.
Don’t taste anything in the laboratory
DO’S AND DON’TS IN A BIOCHEMISTRY LABORATORY:
Wear lab coat (Appron) and shoes while working in the laboratory.
Check and read the label of the reagent bottle carefully before using its content. Read procedures and precautions carefully and follow them.
It is a bad practice to leave the reagent bottles on the working table. Put the stoppers properly on the bottles and keep them on the shelf immediately after use.
If a reagent bottle is empty, ask the laboratory attendant to fill it.
If you require a reagent from the bottle kept on side shelf, take the test tube or the beaker from your working table and take reagent in that and then put the bottle at its respective place on the shelf. Do not bring the bottle to your seat.
Avoid using excessive amounts of reagents unless you are advised to do so.
Use only properly cleaned droppers, spatulas or pipettes etc. to take out the reagents from
the stock solutions and reagent bottles.
Do not keep the stopper of the bottle on the table.
To take out dry solid reagents use spatula and keep it on watch glass, never use filter
papers.
Do not keep the reagent on your palm or touch it with your fingers.
Never throw used match sticks, litmus papers, broken glass apparatus, filter papers or any other insoluble solid material into the sink or on the floor. Dispose them off in the waste bin provided at your working table.
Do not waste water or gas. Close the taps whenever they are not in use. Do not leave the lighted burner under the wire gauze when nothing is being heated. Extinguish it.
