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Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about: * Culture media and vessels * Techniques and procedures for both orchids which were previously cultured and for those which were not * Plant hormones and growth regulators * Media components * Methods for tissue decontamination * Historical information * Procedures for the cultivation for plantlets which have been removed from flasks * Sources of light and illumination methods Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids.
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Cover
Volume I: Micropropagation of Orchids
Title Page
Preface
Preface to the Second Edition
Preface to the First Edition
CHAPTER ONE: History
Terminology
Origins of Orchid Micropropagation
Culture of Tissues and Organs
First Micropropagation of Orchids
The Second Aseptic Culture of an Orchid Explant
Plant Diseases and Meristems
The Third Aseptic Culture of an Orchid Explant
The Fourth Aseptic Culture of an Orchid Explant
Who Pioneered Micropropagation?
Root Cultures
Leaf Cultures
Stems
Flower Buds, Flowers, Floral Segments, and Reproductive Organs
Inflorescences
A Patent
Doubtful Claims
Mutations
Theft in Vitro
Darkening of Culture Media
Anticontaminants
Cell and Protoplast Culture
Flowering In Vitro
Solidifying Agents
The Future
CHAPTER TWO: General Outline of Techniques and Procedures
Media Components
Stock Solutions
State of the Medium
Sterilization
Surface Decontamination
Preparation of the Medium
Culture Vessels
Culture Conditions
Placing Plant Material in Culture
Internal Contaminants
Mites and Thrips as Contaminants and Contamination Vectors
Virus infections and testing
Enzymes for Protoplast Isolation
Work Area
Washing Glassware
Statistics
CHAPTER THREE: Methods for Specific Genera
Acampe
Aeridachnis
Aerides
Aeridovanda
Anacamptis
Angraecum
Anoectochilus
Ansellia
Arachnis
Arachnostylis
Aranda
Aranthera
Arundina
Ascocenda
Ascocentrum
Ascofinetia
Barlia
Bletilla
Brassavola
Brassia
Brassocattleya
Brassolaeliocattleya
Bulbophyllum
Burkillara
Caladenia
Calanthe
Catasetum
Cattleya
Caularthron
Cleisostoma
Clowesia
Coelogyne
Cymbidium
Cypripedium
Cyrtopodium
Dactylorchis
Dactylorhiza
Darwinara
Volume II: Micropropagation of Orchids
Title Page
CHAPTER THREE: Methods for Specific Genera
Dendrobium
Disa
Diuris
Doriella
Doritaenopsis
Doritis
Encyclia
Epidendrum
Epiphronitis
Eria
Erycina
Eulophia
Gastrochilus
Geodorum
Goodyera
Grammatophyllum
Gymnadenia
Habenaria
Hadrolaelia
Hetaeria
Holttumara
Ipsea
Kagawara
Laelia
Laeliocattleya
Liparis
Ludisia (Haemaria)
Luisia
Lycaste
Malaxis
Maxillaria
Miltonia
Mokara
Mormodes
Neofinetia
Neostylis
Neottia
Nervilia
Nigritella
Oberonia
Odontioda
Odontoglossum
Odontonia
Oncidium
Ophrys
Orchis
Otochilus
Pachystoma
Paphiopedilum
Papilionanda
Papilionanthe
Phaius
Phalaenopsis
Volume III: Micropropagation of Orchids
Title Page
CHAPTER THREE: Methods for Specific Genera
Pholidota
Phragmipedium
Pleione
Pogonia
Ponerorchis
Potinara
Pterostylis
Renades
Renanetia
Renantanda
Renanthera
Restrepia
Rhynchostele
Rhynchostylis
Saccolabium
Sarcanthus
Satyrium
Schomburgkia
Serapias
Sophrolaeliocattleya
Spathoglottis
Spiranthes
Tainia
Thelymitra
Thunia
Trudelia
Vanda
Vandofinetia
Vanilla
Vascostylis
Vuylstekeara
Xenikophyton
Zygopetalum
CHAPTER FOUR: Summary
Culture Conditions
Media Components
Contamination by Endophytes
Applications of Orchid Tissue Culture
Flowering
Microclimate
Negative Aspects of Micropropagation
References
APPENDIX ONE: General Information on Supplies, Equipment, Terms, and Reagents
APPENDIX TWO: Sources of Supplies and Equipment
APPENDIX THREE: Some Sites of Interest on the World Wide Web
APPENDIX FOUR: Light
APPENDIX FIVE: Formulary
APPENDIX SIX: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions
APPENDIX SEVEN: Additional Information
APPENDIX EIGHT: Plant Preservative Mixture
General Instructions
Selected Answers from Frequently Asked Questions (FAQ) for a Previous Version of PPM™
Use of PPM™ with Orchid Seeds
Use of PPM in Orchid Tissue Culture
Material Safety Data Sheet
Source of PPM™ and Technical Support
Disclaimer
APPENDIX NINE: Media: Additives, Components, Errors, Modifications, Omissions, Substitutions, Sterilization and More
Errors in Original Formulations
Sterilization
Omissions
Additions
Substitutions
Charcoal
Inconsistencies, Errors, and Change in Recipes as a Result of Use
Conclusion
APPENDIX TEN: Computer Letters and Symbol Codes
APPENDIX ELEVEN: Photographing Cultures in Flasks
Glossary
General Subject Index
End User License Agreement
Chapter 02
TABLE 2‐1. Substitutions of salts in culture media
TABLE 2‐2. Use and substitution of chemicals whose molecules contain waters of hydration
TABLE 2‐3. Some hormones, antihormones, and hormone inhibitors used in orchid micropropagation media
TABLE 2‐4. Composition of banana pulp
TABLE 2‐5. Partial composition of coconut water, copra, and coconut oil (Raghavan, 1966, 1976; Woodroof, 1979; Arditti and Ernst, 1993; Ge et al., 2004, 2005, 2006; de Sousa et al., 2005; Anonymous, 2008
c
, 2008
d
, 2008
e
, 2008
f
)
TABLE 2‐6. Typical analysis of Difco peptones and hydrolysates
TABLE 2‐7. Sugars in orchid tissue culture media
TABLE 2‐8A. Stock solutions of anticontaminants
TABLE 2‐8B. Formulations of anticontaminants for use in culture media for orchid seedlings (Thurston et al., 1979)
TABLE 2‐9. Antibiotics, bactericides, and fungicides which can be useful in plant tissue culture media
TABLE 2‐10. Mass spectrographic analysis of charcoal (Weatherhead, 1979)
TABLE 2‐11. Typical analyses and some characteristics of reagent‐grade agars from three companies
TABLE 2‐12. Illumination in foot candles from two standard cool white fluorescent lamps on a white reflecting surface (Downs et al., 1966) and two Gro Lux tubes (Mpelkas, 1965) measured at several distances
TABLE 2‐13. Conversion of foot candles to W cm
−2
for several light sources (Klein, 1973)
TABLE 2‐14. Conversion factors from lux or foot candles to watts per square meter of photosynthetically active radiation (W m
−2
PAR), or to micromoles of photosynthetic illumination per square meter per second (µmol m
−2
s
−1
PAR) for several light sources (Hartman et al., 1988)
TABLE 2‐15. Production of photosynthetically active radiation by several light sources (Langham, 1978)
TABLE 2‐16. Interconversion between photosynthetically active radiation (PAR) and foot candles
TABLE 2‐17. Light conversion factors
TABLE 2‐18. Light intensity estimations in foot candles using a camera or a light meter
TABLE 2‐19. Some facts about carbon dioxide
TABLE 2‐20. Dilutions of household bleaches containing different concentrations of sodium hypochlorite
TABLE 2‐21. Use of thin layer explants for regeneration, organogenesis, embryogenesis, and transformation in orchids (modified from Teixeira da Silva, 2013
a
)
Chapter 03
TABLE ACAMPE-1. Modified Vacin and Went medium (Vacin and Went, 1949) for protoplast culture of
Acampe praemorsa
(Seeni and Abraham, 1986)
TABLE ACAMPE-2. Heller’s medium (Heller, 1953) as modified for the culture of leaf tips from seedlings of
Acampe rigida
(Yam, 1989)
TABLE ACAMPE-3. Ichihashi–Yamashita medium (Ichihashi and Yamashita, 1977) as modified for the culture of leaf tips from seedlings of
Acampe rigida
(Yam, 1989)
TABLE ACAMPE-4. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Acampe praemorsa
leaf explants (Nayak et al., 1997
a
)
TABLE ACAMPE-5. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified to bring about elongation of
Acampe praemorsa
shoots produced by leaf explants (Nayak et al., 1997a)
TABLE ACAMPE-6. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for rooting
Acampe praemorsa
shoots produced by leaf explants (Nayak et al., 1997
a
)
TABLE ACAMPE-7. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of protocorm-like bodies from leaf explants of
Acampe praemorsa
(Pyati and Murthy, 1999)
TABLE ACAMPE-8. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of callus from leaf explants of
Acampe praemorsa
(Pyati and Murthy, 1999)
TABLE ACAMPE-9. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of callus followed by protocorm-like bodies from leaf explants of
Acampe praemorsa
(Pyati and Murthy, 1999)
TABLE AER-1. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of protocorm-like bodies from leaf explants of
Aerides maculosum
(Murthy and Pyati, 2001)
TABLE AER-2. Basal Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for shoot and root formation on protocorm-like bodies generated from leaf explants of
Aerides maculosum
(Murthy and Pyati, 2001)
TABLE AER-3. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of apical sections of
Aerides multiflorum
leaf explants (Vij and Pathak, 1990)
TABLE AER-4. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of basal explants of
Aerides multiflorum
leaves (Vij and Pathak, 1990)
TABLE AER-5. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of root explants of
Aerides multiflorum
leaf explants (Vij, 1993)
TABLE AER-6. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of protocorm-like body/protocorm explants of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-7. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of leaf explants of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-8. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for plantlet production from protocorm-like bodies of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-9. Basal Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of protocorm-like body/protocorm explants of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-10. Basal Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of leaf explants of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-11. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for plantlet production from protocorm-like bodies of
Aerides crispum
(Sheelavathnath et al., 2005)
TABLE AER-12. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) for the culture of protocorm-like bodies of
Aerides multiflorum
prior to encapsulation and of synseeds (Semdi et al., 2006)
TABLE AER-13. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) for the culture of encapsulated protocorm-like bodies (synseeds) of
Aerides multiflorum
(Semdi et al., 2006)
TABLE AER-14. Sodium alginate containing Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) for the encapsulation of protocorm-like bodies of
Aerides multiflorum
(Semdi et al., 2006)
TABLE AER-15. Calcium chloride solution for the encapsulation of protocorm-like bodies (PLBs) of
Aerides multiflorum
(Sembi et al., 2006)
TABLE ANAC-1. Modified Murashige–Skoog (MS) medium for the culture of
Anacamptis pyramidalis
meristem (Morel, 1970)
TABLE ANGCM-1. Enriched B5 medium (Gamborg et al., 1968) for the culture of
Angraecum giryamae
protoplasts (Price and Earle, 1984)
TABLE ANGCM-2. Protoplast medium (Dudits et al., 1976
a
, 1976
b
) modified for the culture of
Angraecum giryamae
protoplasts (Price and Earle, 1984)
TABLE ANCT-1. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for the culture of lateral buds of
Anoectochilus formosanus
(
Anoectochilus roxburghii
) (Chow et al., 1982)
TABLE ANCT-2. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for rooting of
Anoectochilus formosanus
in vitro (Chow et al., 1982)
TABLE ANCT-3. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for in vitro layering of
Anoectochilus formosanus
(Chow et al., 1982)
TABLE ANCT-4. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for layering of
Anoectochilus formosanus
stems (Huang et al., 1991)
TABLE ANCT-5. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for leaf-bearing stem segments of
Anoectochilus formosanus
(Huang et al., 1991)
TABLE ANCT-6. Woody plants medium (Llyod and McCown, 1980; McCown and Sellmer, 1987) as used for the culture of
Anoectochilus regalis
shoot cuttings (Gangaprasad et al., 2000)
TABLE ANCT-7. Woody plants medium (Llyod and McCown, 1980; McCown and Sellmer, 1987) as used for the culture of
Anoectochilus regalis
shoots (Gangaprasad et al., 2000)
TABLE ANCT-8. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Anoectochilus formosanus
shoot tip and node explants (Wu et al., 2007)
TABLE ASLLA-1. PhytoTechnology P668 medium (www.phytotechlab.com) for seed germination and seedling culture of
Ansellia africana
(Vasudevan and Van Staden, 2011)
TABLE ASLLA-2. PhytoTechnology P668 medium (www.phytotechlab.com) for the culture of nodal segments from 16-week-old seedlings of
Ansellia africana
(Vasudevan and Van Staden, 2011)
TABLE ARACH-1. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for the culture of
Arachnis
buds (Vajrabhaya and Vajrabhaya, 1976
a
)
TABLE ARACH-2. A medium for the culture and proliferation of
Arachnis
buds (Vajrabhaya and Vajrabhaya, 1976
a
)
TABLE ARACH-3. Modified Vacin and Went medium (Vacin and Went, 1949) for initial culture of shoot tips from apical and axillary buds of
Arachnis
(Lim-Ho, 1981)
TABLE ARACH-4. Modified Vacin and Went medium (Vacin and Went, 1949) for the proliferation and differentiation of
Arachnis
explants (Lim-Ho, 1981)
TABLE ARACH-5. Modified Vacin and Went medium (Vacin and Went, 1949) for the proliferation and differentiation of
Arachnis
explants (Lim-Ho, 1981)
TABLE ARACH-6. Modified Vacin and Went medium (Vacin and Went, 1949) for plantlet formation from
Arachnis
explants (Lim-Ho, 1981)
TABLE ARACH-7. Maceration medium for isolation of cells from tissues of
Arachnis
Maggie Oei (Hew and Yip, 1986)
TABLE ARACH-8. Washing medium for isolated cells of
Arachnis
Maggie Oei (Hew and Yip, 1986)
TABLE ARACH-9. Incubation medium for isolated cells of
Aachnis
Maggie Oei (Hew and Yip, 1986)
TABLE ARANDA-1. A medium consisting of the Vacin and Went macroelements (Vacin and Went, 1949), Heller’s microelements (Heller, 1953), chelated iron, and other additives for the culture of shoot-tip and auxillary-bud explants of
Aranda
(Khaw et al., 1978
a
, 1978
b
)
TABLE ARANDA-2. Vacin and Went medium (Vacin and Went, 1949) as used for the culture of
Aranda
explants (Loh et al., 1978; Goh, 1981)
TABLE ARANDA-3. White’s medium (Singh and Krikorian, 1981) as used for the culture of
Aranda
explants (Goh, 1973)
TABLE ARANDA-4. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for the culture of
Aranda
leaf explants (Fu, 1978, 1979
a
, 1979
b
)
TABLE ARANDA-5. Liquid Vacin and Went medium (Vacin and Went, 1949) as used for the maintenance of protocorm-like bodies of
Aranda
(Fu, 1978, 1979
a
, 1979
b
)
TABLE ARANDA-6. Vacin and Went medium (Vacin and Went, 1949) as used for plantlet regeneration from protocorm-like bodies derived from
Aranda
leaves (Fu, 1978, 1979
a
, 1979
b
)
TABLE ARANDA-7. Knudson C medium (Knudson, 1946)
TABLE ARANDA-8. Modified Knudson C medium (Knudson, 1946)
TABLE ARANDA-9. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the differentiation of
Aranda
explants (Fu, 1978, 1979
a
, 1979
b
)
TABLE ARANDA-10. Knudson C medium (Knudson, 1946) modified for root induction on plantlets of Aranda (Fu, 1978, 1979
a
, 1979
b
)
TABLE ARANDA-11. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for initial culture of
Aranda
leaves (Lim-Ho, 1981)
TABLE ARANDA-12. Vacin and Went macroelements (Vacin and Went, 1949) supplemented for the culture of mesophyll protoplasts of
Aranda
Noorah Alsagoff (Loh and Rao, 1985)
TABLE ARANDA-13. Vacin and Went macroelements (Vacin and Went, 1949) supplemented for the culture of mesophyll protoplasts of
Aranda
Noorah Alsagoff (Loh and Rao, 1985)
TABLE ARANDA-14. Kao–Wetter 8p medium (Kao and Wetter, 1977) modified for the culture of
Aranda
protoplasts (Koh et al., 1988)
TABLE ARANDA-15. Relation between carbon–nitrogen ratio, formation of protocorm-like-bodies, and chlorophyll content in callus of
Aranda
Tay Swee Eng (Chia et al., 1988)
TABLE ARANDA-16. Knudson C medium (Knudson, 1946) as modified for the culture of
Aranda
Deborah inflorescence tips (Goh and Wong, 1990)
TABLE ARANDA-17. Vacin and Went medium (Vacin and Went, 1949) modified for the culture of protocorm-like bodies derived from inflorescence-shoot-tip explants of
Aranda
Deborah (Goh and Wong, 1990)
TABLE ARANDA-18. Vacin and Went medium (Vacin and Went, 1949) modified for the culture of protocorm-like bodies derived from inflorescence-shoot-tip explants of
Aranda
Deborah (Goh and Wong, 1990)
TABLE ARANDA-19. Vacin and Went medium (Vacin and Went, 1949) modified for the culture of thin sections of
Aranda
Debora shoot tips and protocorm-like body proliferation (Laksmanan et al., 1995)
TABLE ARANDA-20. Vacin and Went medium (Vacin and Went, 1949) modified for differentiation and growth of plantlets produced by protocorm-like bodies (PLBs) derived from thin sections of
Aranda
Deborah shoot tips (Lakshmanan et al., 1995)
TABLE ARNTH-1. Modified Knudson C medium (Knudson, 1946) for the culture of
Aranthera
James Storie buds (Irawati et al., 1977)
TABLE ARNTH-2. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of buds of
Aranthera
James Storie (Irawati et al., 1977)
TABLE ARNTH-3. Vacin and Went medium (Vacin and Went, 1949) for the initial culture of apical and axillary buds of
Aranthera
James Storie (Irawati et al., 1977)
TABLE ARNTH-4. Modified Vacin and Went medium (Vacin and Went, 1949) for the rapid multiplication of protocorm-like bodies produced by apical and axillary buds of
Aranthera
James Storie (Cheah and Sagawa, 1978)
TABLE ARNTH-5. Vacin and Went medium (Vacin and Went, 1949) for shoot production from protocorm- like bodies of
Aranthera
James Storie (Cheah and Sagawa, 1978)
TABLE ARNTH-6. Vacin and Went medium (Vacin and Went, 1949) for root induction on shoots of
Aranthera
James Storie (Cheah and Sagawa, 1978)
TABLE ARNTH-7. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of apical meristems of
Aranthera
James Storie (Ong and Chua, 1980)
TABLE ARNTH-8. Modified Vacin and Went medium (Vacin and Went, 1949) for the proliferation of protocorm-like bodies and plantlet production of
Aranthera
James Storie (Ong and Chua, 1980)
TABLE ARNTH-9. Vacin and Went medium (Vacin and Went, 1949) modified for initial culture of
Aranthera
leaves (Lim-Ho, 1981)
TABLE ARND-1. Effects of media used in the culture of shoot-tip and stem sections of
Arundina bambusifolia
(Mitra, 1971)
TABLE ARND-2. Raghavan and Torrey (1964) medium as used for the culture of
Arundina bambusifolia
6–8-mm-long shoot tips and stem disks (Mitra, 1971)
TABLE ARND-3. A medium (Raghavan and Torrey, 1964) modified for the culture of 1–2-mm-long stem tips of
Arundina bambusifolia
(Mitra, 1971)
TABLE ARND-4. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Arundina bambusifolia
shoot tips (Banik et al., 1986)
TABLE ARND-5. Vacin and Went medium (Vacin and Went, 1949) modified for seed germination, and the culture of thin stem sections and encapsulated protocorms of
Arundina graminifolia
(Kanjilal et al., 2000 and unpublished)
TABLE ARND-6. Half strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Arundina graminifolia
stem segments (Martin, 2007)
TABLE ARND-7. Half strength Murashige-Skoog (MS) medium (Murachige and Skoog, 1962) modified for the culture of
Arundina graminifolia
stem segments (Martin, 2007)
TABLE ARND-8. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Arundina graminifolia
growing buds and protocorm-like bodies (PLB) proliferation (Martin, 2007)
TABLE ARND-9. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Arundina graminifolia
shoots (Martin, 2007)
TABLE ARND-10. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the rooting of
Arundina graminifolia
shoots (Martin, 2007)
TABLE ARND-11. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the maintenance of
Arundina graminifolia
seedlings (Bhadra and Bhowmik, 2005
b
)
TABLE ARND-12. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of seedling rhizome explants of
Arundina graminifolia
(Bhadra and Bhowmik, 2005
b
)
TABLE ARND-13. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the elongation of shoots produced by seedling rhizome explants of
Arundina graminifolia
(Bhadra and Bhowmik, 2005
b
)
TABLE ARND-14. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of seedlings and plantlets of
Arundina graminifolia
(Bhadra and Bhowmik, 2005
b
)
TABLE ASCDA-1. Mitra, Prasad, and Roychowdhury medium (Mitra et al., 1976) as modified for the culture of leaf explants of
Ascocenda
Fiftieth State Beauty (Vij and Kaur, 1999)
TABLE ASCTM-1. Vacin and Went (VW) medium (Vacin and Went, 1949) with the microelements of the Murashige–Skoog (MS) solution (Murashige and Skoog, 1962) as modified for the culture of
Ascocentrum ampullaceum
protocorm-like bodies, and referred to as the WS medium (Wannakrairoj and Tanyasonti, 1996).
TABLE ASCF-1. Effects of four culture media on inflorescence explants and tissues of
Ascofinetia
,
Neostylis
, and
Vascostylis
(Intuwong and Sagawa, 1973)
TABLE ASCF-2. Modified Vacin and Went medium for the culture of
Ascofinetia
inflorescences (Intuwong and Sagawa, 1973)
TABLE BLE-1. Murashige–Skoog medium (Murashige and Skoog, 1962) as modified for the culture of root tips of
Bletilla striata
(Yam, 1989)
TABLE BLE-2. Knudson C medium for the germination of orchid seeds (Knudson, 1946) as modified for the culture of root-tip-derived plantlets of
Bletilla striata
(Yam, 1989)
TABLE BLE-3. Knop’s medium (Knop, 1884) modified for the culture of
Dendrobium
stem nodes and used to culture stem nodes of
Bletilla striata
(Yam, 1989)
TABLE BLE-4. Modified Ichihashi (MI) medium (Ichihashi, 1989) as used for the culture of
Bletilla striata
sections of young pseudobulbs (Vij and Dhiman, 1997)
TABLE BLE-5. Modified Ichihashi (MI) medium (Ichihashi, 1989) as used for the culture of
Bletilla striata
plantlets obtained from sections of young pseudobulbs (Vij and Dhiman, 1997)
TABLE BC-1. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for the culture of
Brassocattleya
buds (Kako, 1973)
TABLE BC-2. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for callus induction from buds of
Brassocattleya
(Kako, 1973)
TABLE BC-3. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of axillary buds of
Brassocattleya
Pastoral “Innocense” (Kishi and Takagi, 1997)
TABLE BC-4. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for plantlet production from protocorm-like bodies of
Brassocattleya
Pastoral ‘Innocense’ (Kishi and Takagi, 1997)
TABLE BULB-1. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the culture of
Bulbophyllum
(Vij et al., 2000
a
)
TABLE CAL-1. B5 medium (Gamborg et al., 1968) modified for the induction of protocorm-like bodies from apical meristems of
Calanthe discolor
buds (Ichihashi, 1994)
TABLE CAL-2. B5 medium (Gamborg et al., 1968) modified for callus induction from apical meristems of
Calanthe discolor
buds (Ichihashi, 1994)
TABLE CAL-3. B5 medium (Gamborg et al., 1968) modified for the induction of multiple shoots and roots on tissues or protocorm-like bodies derived from meristems of
Calanthe discolor
buds (Ichihashi, 1994)
TABLE CAL-4. Isolation medium for
Calanthe
protoplasts (Yasugi et al., 1986)
TABLE CAL-5. Modified B5 medium (Gamborg et al., 1968) modified for the culture of
Calanthe aristulifera
shoot meristetms and containing benzyl adenine (Yamamoto et al., 2012)
TABLE CAL-6. Modified B5 medium (Gamborg et al., 1968) modified for the culture of
Calanthe striata
shoot meristems and containing benzyl adenine (Yamamoto et al., 2012)
TABLE CAL-7. Modified B5 medium (Gamborg et al., 1968) modified for the possible subculture of
Calanthe aristulifera
protocorm-like bodies and free of benzyladenine (Yamamoto et al., 2012)
TABLE CAL-8. Modified B5 medium (Gamborg et al., 1968) modified for the possible subculture of
Calanthe striata
protocorm-like bodies and free of benzyladenine (Yamamoto et al., 2012)
TABLE CTSM-1. Effect of culture media on
Catasetum
root tips (Kerbauy, 1984
a
)
TABLE CTSM-2. Knudson C medium (Knudson, 1946) as modified for the culture of
Catasetum
root tips (Kerbauy, 1984
a
)
TABLE CTSM-3. Knudson C medium (Knudson, 1946) as modified for the production of callus and protocorm-like bodies from
Catasetum
root (Kerbauy, 1984)
TABLE CTSM-4. Knudson C medium (Knudson, 1946) as modified for the protocorm and plantlet production from root tips of
Catasetum
root tips (Kerbauy, 1984
a
)
TABLE CTSM-5. Linsmaier–Skoog (LS) medium (Linsmaier and Skoog, 1965) modified for the culture of
Catasetum fimbriatum
root tips (Colli and Kerbauy, 1993)
TABLE CTSM-6. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of
Catasetum pileatum
root tips (Kraus and Monteiro, 1989; Kraus and Kerbauy, 1992)
TABLE C-1. Starting medium for the culture of
Cattleya
explants (Lindemann et al., 1970)
TABLE C-2. Standard maintenance medium for proliferating callus cultures of
Cattleya
(Lindemann et al., 1970)
TABLE C-3. Modified Knudson C (Knudson, 1946) medium for plantlet formation and root induction in
Cattleya
TABLE C-4. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for the culture of
Cattleya
buds
TABLE C-5. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cattleya
buds
TABLE C-6. Modified Knudson C medium (Knudson, 1946) for the culture of
Cattleya
shoot meristems (Morel, 1965
a
, 1965
b
, 1970)
TABLE C-7. Modified Knudson C medium (Knudson, 1946) for the culture of
Cattleya
buds
TABLE C-8. Reinert–Mohr medium (Reinert and Mohr, 1967) for the initial culture of
Cattleya
bud meristems
TABLE C-9. Solid medium for subculture of
Cattleya
bud meristems (Reinert and Mohr, 1967)
TABLE C-10. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of explants from vegetative shoots of
Cattleya
(Scully, 1967)
TABLE C-11. Modified Knudson C medium (Knudson, 1946) for the culture of dormant buds of
Cattleya
(Vajrabhaya, 1978)
TABLE C-12. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for use as the starting solution in the culture of
Cattleya
explants (Huang, 1984)
TABLE C-13. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for the culture of
Cattleya
shoots (Huang, 1984)
TABLE C-14. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the rooting of
Cattleya
shoots (Huang, 1984)
TABLE C-15. Culture medium for
Cattleya
leaf bases (Champagnat et al., 1970)
TABLE C-16. Modified Heller’s medium (Heller, 1953) for the culture of tips from young leaves of mature
Cattleya
plants (Churchill et al., 1971
a
)
TABLE C-17. Murashige–Skoog medium (Murashige and Skoog, 1962) as used for the maintenance of callus cultures derived from
Cattleya
leaf tips (Churchill et al., 1971)
TABLE C-18. Linsmaier–Skoog medium (Linsmaier and Skoog, 1965) as used for the maintenance of callus cultures derived from
Cattleya
leaf tips (Churchill et al., 1971
a
)
TABLE C-19. Modified Knudson C medium (Knudson, 1946) for the differentiation of plantlets from
Cattleya
leaf callus (Churchill et al., 1971
a
)
TABLE C-20. Mitra, Prasad, and Roychowdhury medium (Mitra et al., 1976) as modified for the culture of root tips of
Cattleya
Almakee (Vij, 1993)
TABLE C-21. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of proliferated
Cattleya mossiae
explants (Huang, 1984; Torres and Mogollón, 1998, 2000)
TABLE C-22. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of proliferated
Cattleya mossiae
explants (Huang, 1984; Torres and Mogollón, 1998, 2000)
TABLE C-23. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of root-tip explants from
Cattleya nobilior
×
Cattleya loddigesii
seedlings (Kerbauy, 1991)
TABLE C-24. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the germination of
Cattleya nobilior
×
Cattleya loddigesii
seeds (Kerbauy, 1991)
TABLE C-25. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for the culture of shoot-tip explants of
Cattleya leopoldii
(Saiprasad et al., 2002
a
)
TABLE C-26. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for plantlet production of shoot-tip explants derived from protocorm-like bodies (PLBs) of
Cattleya leopoldii
(Saiprasad et al., 2002
a
)
TABLE C-27. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of shoot formation by
Cattleya
explants (Cybularz-Urban and Hanus-Fajerska, 2006)
TABLE C-28. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for propagation of shoots produced by
Cattleya
explants (Cybularz-Urban and Hanus-Fajerska, 2006)
TABLE C-29. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for induction of shoot formation by
Cattleya
explants (Cybularz-Urban and Hanus-Fajerska, 2006)
TABLE C-30. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for propagation of shoots produced by
Cattleya
explants (Cybularz-Urban and Hanus-Fajerska, 2006)
TABLE C-31. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for propagation of shoots produced by
Cattleya
explants (Cybularz-Urban and Hanus-Fajerska, 2006)
TABLE C-32. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for shoot regeneration in
Narcissus
(Kozak, 1991) as used for the culture of a hybrid described as
Cattleya waltersiana
×
Cattleya marone
(Cybularz-Urban and Bach, 2007) which does not exist and may be
Cattleya
Eros (
Cattleya walkeriana
×
Cattleya mossiae
).
TABLE CLOWESIA-1. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of root-tip explants from
Clowesia warscewiczii
seedlings (Kerbauy, 1996)
TABLE COEL-1. Basal Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for the germination of
Coelogyne stricta
seeds (Basker and Bai, 2006)
TABLE COEL-2. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Coelogyne stricta
pseudobulb explants from 1-year-old seedlings (Basker and Bai, 2006)
TABLE COEL-3. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the rooting of
Coelogyne stricta
shoots derived from pseudobulb explants (Basker and Bai, 2006)
TABLE COEL-4. Hyponex medium for the germination of
Coelogyne cristata
seeds (Naing et al., 2010)
TABLE COEL-5. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Coelogyne cristata
seedlings (Naing et al., 2010)
TABLE COEL-6. Half-strength Murashige–Skoog (hMS) medium (Murashige and Skoog, 1962) modified for the culture of shoot axis explants from
Coelogyne cristata
seedlings (Naing et al., 2010)
TABLE COEL-7. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for shoot induction on intact seedlings of
Coelogyne cristata
seedlings (Naing et al., 2010)
TABLE COEL-8. Half-strength Murashige–Skoog (hMS) medium (Murashige and Skoog, 1962) modified for the culture of pseudobulb explants from
Coelogyne cristata
seedlings (Naing et al., 2010)
TABLE CYM-1. Modified Knudson C medium (Knudson, 1946) for plantlet formation from
Cymbidium
shoot-tip meristems (Morel, 1965
a
, 1965
b
, 1970)
TABLE CYM-2. Modified Knudson C medium (Knudson, 1946) for plantlet production from
Cymbidium
shoot-tip meristems (Morel, 1965
a
, 1965
b
, 1970)
TABLE CYM-3. Modified Knudson C medium (Knudson, 1946) for the differentiation of plantlets from leaf-tip callus (Arditti et al., 1971, 1972; Ball et al., 1971; Churchill et al., 1971
a
, 1971
b
, 1971
c
, 1972
b
, 1973) which is suitable for
Cymbidium
shoot-tip explants
TABLE CYM-4. Culture medium for
Cymbidium
shoot tips (Wimber, 1963)
TABLE CYM-5. Modified Vacin and Went medium for the culture of
Cymbidium
shoot-tip explants (Sagawa et al., 1966)
TABLE CYM-6. Medium for the in vitro propagation of
Cymbidium
(Fonnesbech, 1972
a
, 1972
b
)
TABLE CYM-7. White’s medium (Singh and Krikorian, 1981) modified for the culture of
Cymbidium ensifolium
shoot tips (Wang et al., 1981)
TABLE CYM-8. White’s medium (Singh and Krikorian, 1981) modified for the culture of
Cymbidium ensifolium
shoot tips (Wang et al., 1981)
TABLE CYM-9. White’s medium (Singh and Krikorian, 1981) modified for the culture of
Cymbidium ensifolium
shoot tips (Wang et al., 1981)
TABLE CYM-10. White’s medium (Singh and Krikorian, 1981) for the culture of
Cymbidium ensifolium
(Wang et al., 1981; Wang, 1984, 1988
a
, 1988
b
, no date
a
, no date
b
)
TABLE CYM-11. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of protocorm-like bodies derived from
Cymbidium ensifolium
TABLE CYM-12. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for flower induction in
Cymbidium ensifolium
in vitro (Wang, no date
a
)
TABLE CYM-13. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium goeringii
in vitro (Wang, no date
a
)
TABLE CYM-14. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium goeringii
in vitro (Wang, no date
a
)
TABLE CYM-15. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium goeringii
in vitro (Wang, no date
a
)
TABLE CYM-16. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium goeringii
in vitro (Wang, no date
a
)
TABLE CYM-17. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium goeringii
in vitro (Wang, no date
a
)
TABLE CYM-18. Knudson C medium (Knudson, 1946) modified for root induction in
Cymbidium goeringii
TABLE CYM-19. Murashige–Skoog medium (Murashige and Skoog, 1962) for the culture of dormant buds of
Cymbidium
(Tran Thanh Van, 1974)
TABLE CYM-20. Murashige–Skoog medium (Murashige and Skoog, 1962) for plantlet productionfrom protocorm-like bodies derived from dormant buds of
Cymbidium
(Tran Thanh Van, 1974)
TABLE CYM-21. Knudson C medium (Knudson, 1946) modified for the culture of meristematic tissues from
Cymbidium
buds (Dalla Rosa and Laneri, 1977)
TABLE CYM-22. Vacin and Went medium (Vacin and Went, 1949) modified for
Cymbidium
shoot tip cultures (Gu et al., 1987
b
)
TABLE CYM-23. Vacin and Went medium (Vacin and Went, 1949) modified for plantlet production from
Cymbidium
protocorm-like bodies (Gu et al., 1987
b
)
TABLE CYM-24. Knudson C medium (Knudson, 1946) modified for tissue culture of
Cymbidium
(van Rensburg and Vcelar, 1984)
TABLE CYM-25. Knudson C medium (Knudson, 1946) modified for
Cymbidium
culture in vitro (Kusumoto and Furukawa, 1977; Kusumoto, 1978, 1980
a
, 1980
b
, 1981
a
)
TABLE CYM-26. White’s medium (Singh and Krikorian, 1981) for the culture of
Cymbidium
shoot tips (Steward and Mapes, 1971
a
)
TABLE CYM-27. White’s medium (Singh and Krikorian, 1981) for the production of free cells from callus cultures derived from
Cymbidium
shoot tips (Steward and Mapes, 1971
a
)
TABLE CYM-28. White’s medium (Singh and Krikorian, 1981) for the production of free cells from callus cultures derived from
Cymbidium
shoot tips (Steward and Mapes, 1971
a
)
TABLE CYM-29. Modified Kyoto medium as used for initial culture of rhizome tips of
Cymbidium faberi
(Hasegawa et al., 1985)
TABLE CYM-30. Modified Kyoto medium as used for the growth of shoots derived from rhizome tips of
Cymbidium faberi
(Hasegawa et al., 1985)
TABLE CYM-31. Nitsch and Nitsch (NN) medium (Nitsch and Nitsch, 1969) as used for the culture of
Cymbidium aloifolium
shoot tips (Devi et al., 1997)
TABLE CYM-32. Basal Nitsch and Nitsch (NN) medium (Nitsch and Nitsch, 1969) as used for hardening plantlets produced through the culture of
Cymbidium aloifolium
shoot tips (Devi et al., 1997)
TABLE CYM-33. Half-strength basal Nitsch and Nitsch (½NN) medium (Nitsch and Nitsch, 1969) as used for hardening plantlets produced through the culture of
Cymbidium aloifolium
shoot tips (Devi et al., 1997)
TABLE CYM-34. Quarter-strength basal Nitsch and Nitsch (¼NN) medium (Nitsch and Nitsch, 1969) as used for hardening plantlets produced through the culture of
Cymbidium aloifolium
shoot tips (Devi et al., 1997)
TABLE CYM-35. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for the culture of
Cymbidium mastersii
root-tip explants (Vij, 1993, 1994)
TABLE CYM-36. Mitra, Prasad, and Roychowdhury medium (Mitra et al., 1976) as modified for the culture of root tips of
Cymbidium pendulum
(Vij, 1993)
TABLE CYM-37. Mitra, Prasad, and Roychowdhury medium (Mitra et al., 1976) modified for the culture of shoot tips of
Cymbidium pendulum
(Vij et al., 1994
a
)
TABLE CYM-38. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium giganteum
shoot tips (Corrie and Tandon, 1993)
TABLE CYM-39. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of encapsulated protocorm-like bodies of
Cymbidium giganteum
(Corrie and Tandon, 1993)
TABLE CYM-40. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the encapsulation of protocorm-like bodies of
Cymbidium giganteum
(Corrie and Tandon, 1993)
TABLE CYM-41. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for theencapsulation of protocorm-like bodies of
Cymbidium giganteum
(Corrie and Tandon, 1993)
TABLE CYM-42. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified forthe culture of
Cymbidium goeringii
flower bud explants (Shimasaki and Uemoto, 1991)
TABLE CYM-43. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for plantlet production from
Cymbidium goeringii
rhizomes derived from flower bud explants (Shimasaki and Uemoto, 1991)
TABLE CYM-44. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of shoot explants of
Cymbidium aloifolium
(Nayak et al., 1997
b
)
TABLE CYM-45. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of new shoots from shoot explants of
Cymbidium aloifolium
(Nayak et al., 1997
b
)
TABLE CYM-46. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the rooting of
Cymbidium aloifolium
shoots obtained from shoot tips in vitro (Nayak et al., 1997
b
)
TABLE CYM-47. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of shoots of
Cymbidium aloifolium
(Nayak et al., 2002)
TABLE CYM-48. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of thin cross-section explants of
Cymbidium aloifolium
(Nayak et al., 2002)
TABLE CYM-49. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the rooting of shoots produced by thin cross-section explants of
Cymbidium aloifolium
(Nayak et al., 2002)
TABLE CYM-50. Liquid-modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for proliferation of protocorm-like bodies derived from shoot tips (Begum et al., 1994
a
)
TABLE CYM-51. Liquid-modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for the culture of epidermal cell peels (outer tissue) of
Cymbidium
(Begum et al., 1994
a
)
TABLE CYM-52. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of and plantlet production from protocorm-like bodies produced by epidermal (outer) layers of
Cymbidium
(Begum et al., 1994
a
)
TABLE CYM-53. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of
Cymbidium ensifolium
var.
misericors
explants (Chang and Chang, 1998)
TABLE CYM-54. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the maintenance of
Cymbidium ensifolium
var.
misericors
callus (Chang and Chang, 1998)
TABLE CYM-55. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the induction of globular granule masses from callus of
Cymbidium ensifolium
var.
misericors
(Chang and Chang, 1998)
TABLE CYM-56. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for rhizome induction from globular granules of
Cymbidium ensifolium
var.
misericors
(Chang and Chang, 1998)
TABLE CYM-57. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for plantlet production of
Cymbidium ensifolium
var.
misericors
explants (Chang and Chang, 1998)
TABLE CYM-58. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of
Cymbidium ensifolium
var.
misericors
rhizome explants (Chang and Chang, 2000
a
)
TABLE CYM-59. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for plantlet production from
Cymbidium ensifolium
var.
misericors
rhizome explants (Chang and Chang, 2000
a
)
TABLE CYM-60. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for proliferation of explants from
Cymbidium sinense
seed-derived rhizomes (Chang and Chang, 2000
b
)
TABLE CYM-61. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for vegetative (shoot) bud induction on
Cymbidium sinense
rhizome explants (Chang and Chang, 2000
b
)
TABLE CYM-62. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for plantlet production from buds on
Cymbidium sinense
rhizome explants (Chang and Chang, 2000b)
TABLE CYM-63. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for plantlet production from seedling-derived rhizomes (Nayak et al., 1998)
TABLE CYM-64. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the proliferation of
Cymbidium niveo-marginatum
rhizomes (Kostenyuk et al., 1999).
TABLE CYM-65. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the proliferation of
Cymbidium niveo-marginatum
rhizomes (Kostenyuk et al., 1999).
TABLE CYM-66. Half-strength Murahige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium ensifolium
shoot-tip and axillary bud explants (Wang, 1990)
TABLE CYM-67. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for proliferation of plantlets and shoot production from
Cymbidium ensifolium
protocorm-like bodies obtained from shoot-tip and axillary bud explants (Wang, 1990)
TABLE CYM-68. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as used for the culture of
Cymbidium ensifolium
plantlets which flower in vitro (Wang, 1990)
TABLE CYM-69. Effects of the state of the medium on the growth of protocorm-like bodies and plantlets
TABLE CYM-70. Number of virus-free plants as a function of the number of subcultures and time in ribavirin-containing medium
TABLE CYM-71. Ribavirin-containing Knudson C (KC) medium (Knudson, 1946) for the eradication of virus from protocorm-like bodies of
Cymbidium
(Toussaint et al., 1993)
TABLE CYM-72. Knudson C (KC) medium (Knudson, 1946) for plantlet production from protocorm-like bodies of
Cymbidium
that have been freed from
Odontoglossum
ringspot virus (Toussaint et al., 1993)
TABLE CYM-73. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for virus eradication from
Cymbidium
(Paek et al., 1997)
TABLE CYM-74. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium kanran
rhizome explants (Shimasaki, 1995)
TABLE CYM-75. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Cymbidium kanran
shoots and possible root induction (Shimasaki, 1995)
TABLE CYM-76. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for the culture of rhizome tips of
Cymbidium kanran
(Shimasaki, 1995)
TABLE CYM-77. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of rhizome tips of
Cymbidium ensifolium
and
Cymbidium kanran
(Ogura and Okubo, 2003)
TABLE CYM-78. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for flower induction in
Cymbdium ensifolium
var.
misericors
in vitro (Chang and Chang, 2003).
TABLE CYM-79. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of
Cymbidium aloifolium
seedling node explant and plantlet elongation (Barua and Bhadra, 1999)
TABLE CYM-80. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the rooting of
Cymbidium aloifolium
plants derived from seedling node explants (Barua and Bhadra, 1999)
TABLE CYM-81. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) modified for the culture of
Cymbidium elegans
explants, protocorm-like bodies and shoots (Malabadi and Nataraja, 2007)
TABLE CYM-82. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) modified for the rooting of
Cymbidium elegans
shoots (Malabadi and Nataraja, 2007)
TABLE CYM-83. The vitamins of the Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976)
TABLE CYM-84. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for shoot formation from protocorm-like bodies of
Cymbidium
Maureen Carter (Ogura-Tsujita and Okubo, 2006
a
)
TABLE CYM-85. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of rhizome explants of
Cymbidium kanran
(Ogura-Tsujita and Okubo, 2006
b
) (
Source
: Sasayama and Nagano, 1961.)
TABLE CYM-86. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for shoot formation from rhizome explants of
Cymbidium kanran
(Ogura-Tsujita and Okubo, 2006
b
) (
Source
: Sasayama and Nagano, 1961.)
TABLE CYM-87. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of protocorm-like bodies (PLBs) of
Cymbidium
Twilight Moon ‘Day Light’ (Huan et al., 2004)
TABLE CYM-88. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of protocorm-like body (PLB) explants and callus of
Cymbidium
Twilight Moon ‘Day Light’ (Huan et al., 2004)
TABLE CYM-89. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for protocorm-like body (PLB) production from callus of
Cymbidium
Twilight Moon ‘Day Light’ and rooted plant generation from PLBs (Huan et al., 2004)
TABLE CYM-90. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the maintenance of protocorm-like bodies (PLBs) and the culture of explants from bisected PLBs (Teixeira da Silva and Tanaka, 2006)
TABLE CYM-91. Modified Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of secondary protocorm-like bodies (PLBs), callus induction from bisected PLBs, and callus culture (Teixeira da Silva and Tanaka, 2006)
TABLE CYM-92. Modified Vacin and Went (VW) medium (Vacin and Went, 1949) for callus culture (Teixeira da Silva and Tanaka, 2006)
TABLE CYM-93. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for irrigation of
Cymbidium
plantlets in rockwool (Teixeira da Silva and Tanaka, 2006)
TABLE CYM-94. Hyponex medium for irrigation of
Cymbidium
plantlets in rockwool (Teixeira da Silva and Tanaka, 2006)
TABLE CYM-95. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the germination of
Cymbidium iridioides
seeds (Pant et al., 2009)
TABLE CYM-96. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for shoot induction on explants from in vitro seedlings of
Cymbidium iridioides
(Pant et al., 2009)
TABLE CYM-97. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for shoot induction on explants from in vitro seedlings of
Cymbidium iridioides
(Pant et al., 2009)
TABLE CYM-98. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for rooting of shoots produced by explants from in vitro seedlings of
Cymbidium iridioides
(Pant et al., 2009)
TABLE CYM-99. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) modified for the germination of
Cymbidium giganteum
(Hossain et al., 2010)
TABLE CYM-100. Mitra, Prasad and Roychowdhury medium (Mitra et al., 1976) modified for the rooting of
Cymbidium giganteum
seedlings and shoots (Hossain et al., 2010)
TABLE CYM-101. Knudson C (KC) medium (Knudson, 1946) modified for the culture of thin cross-sections of
Cymbidium
Sleeping Nymph protocorm-like bodies (Vyas et al., 2010)
TABLE CYM-102. Mitra, Prasad and Roychowdhury (MPR) medium (Mitra et al., 1976) modified for the culture of
Cymbidium bicolor
thin-layer explants (Malabadi et al., 2008
a
)
TABLE CYM-103. Mitra, Prasad and Roychowdhury (MPR) medium (Mitra et al., 1976) modified for root induction on shoots of
Cymbidium bicolor
thin-layer explants (Malabadi et al., 2008
a
)
TABLE 1. The growth and developmental response of hybrid
Cymbidium
Twilight Moon ‘Day Light’ half-PLBs to phloroglucinol after 60 days in culture (Teixeira da Silva et al., 2013)
TABLE 2. Root growth and development of hybrid
Cymbidium
Twilight Moon ‘Day Light’ in response to phloroglucinol after 60 days in culture (Teixeira da Silva, 2013
b
)
TABLE 1. The growth and developmental response of hybrid
Cymbidium
Twilight Moon ‘Day Light’ half-PLBs to antioxidants (ascorbic acid and α-tocopherol) and activated charcoal after 60 d in culture on agar-based TC medium (Teixeira da Silva, 2013
c
)
TABLE 2. The growth and developmental response of hybrid
Cymbidium
Twilight Moon ‘Day Light’ half-PLBs cultured on paper bridges to optimized levels (see Table 1) of antioxidants and activated charcoal after 60 d in culture (Teixeira da Silva, 2013
c
)
TABLE 1. Effect of activated charcoal concentrations on rhizome proliferation and growth of
C. sinense
after 50 days of continuous immersion bioreactor culture
TABLE 2. Effect of inoculation densities on rhizome proliferation and growth of
C. sinense
after 50 days of continuous immersion bioreactor culture
TABLE 3. Effect of air volume on rhizome proliferation and growth of
C. sinense
after 50 days of continuous immersion bioreactor culture
TABLE 4. Effect of BA and NAA on shoot formation from rhizome of
C. sinense
after 40 days of temporary immersion bioreactor culture
TABLE 6. Effect of NAA concentrations on plantlet regeneration of
C. sinense
after 50 days of temporary immersion bioreactor culture
TABLE 1. Effect of HA9, chitosan H and BA on organogenesis in PLB culture of
Cymbidium
Waltz ‘Idol’ (Kaewjampa et al., 2012)
TABLE CYP-1. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for culture of root tips of
Cypripedium calceolus
(Tomita and Ramsay, 1998)
TABLE CYP-2. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for culture of root-tip-derived callus, shoots, and plantlets of
Cypripedium calceolus
(Tomita and Ramsay, 1998)
TABLE CYP-3. Tsutsui and Tomita (T) medium (Tsutsui and Tomita, 1990) for immature seed germination and callus induction in
Cypripedium macranthos
var.
taiwanianum
(Tomita and Tomita, 1997)
TABLE CYP-4. Tsutsui and Tomita (T) medium (Tsutsui and Tomita, 1990) for plantlet production from seed-derived callus of
Cypripedium macranthos
var.
taiwanianum
(Tomita and Tomita, 1997)
TABLE CYP-5. Basal Tsutsui and Tomita (T) medium (Tsutsui and Tomita, 1990) for irrigating plantlets of
Cypripedium macranthos
var.
taiwanianum
cultured in sand (Tomita and Tomita, 1997)
TABLE CYP-6. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture Wof
Cypripedium montanum
nodes (Hoshi et al., 1994)
TABLE CYP-7. JA medium (Jo et al., 2001) for culture of seedlings and plantlets of
Cypripedium yatabeanum
(Jo et al., 2001)
TABLE CYP-8. B5 medium (Gamborg et al., 1968) modified for callus induction from root-tip explants of
Cypripedium yatabeanum
(Jo et al., 2001)
TABLE CYP-9. Kao and Michayluck (KM81) medium (Kao and Michayluk, 1981) modified for proliferation of callus derived from root-tip explants of
Cypripedium yatabeanum
(Jo et al., 2001)
TABLE CYP-10. B5 medium (Gamborg et al., 1968) for the culture of of proliferated callus derived from shoot tips of
Cypripedium yatabeanum
(Jo et al., 2001)
TABLE CYP-11. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for the germination of immature seeds of
Cypripedium formosanum
from green (unripe) 3-month-old fruits (Lee and Lee, 2003)
TABLE CYP-12. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for callus production from protocorms produced by immature seeds of
Cypripedium formosanum
(Lee and Lee, 2003)
TABLE CYP-13. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for protocorm-like body (PLB) production from callus produced by immature seeds of
Cypripedium formosanum
(Lee and Lee, 2003)
TABLE CYP-14. Modified Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for plantlet production from protocorm-like bodies derived from callus generated from immature seeds of
Cypripedium formosanum
(Lee and Lee, 2003)
TABLE CYP-15. Modified Van Waes and Debergh medium (VWD) for the culture of rhizomes of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-16. Charcoal-containing modified Van Waes and Debergh medium (VWD) for root induction on shoots of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-17. Modified Van Waes and Debergh medium (VWD) for callus formation on bases of shoots of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-18. Modified Van Waes and Debergh medium (VWD) for the culture of rhizomes of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-19. Modified Van Waes and Debergh medium (VWD) for the culture of rhizomes of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-20. Modified Van Waes and Debergh medium (VWD) for the culture of rhizomes of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-21. Modified Van Waes and Debergh medium (VWD) for the culture of rhizomes of
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-22. Modified Van Waes and Debergh medium (VWD) for the culture of the
Cypripedium
Gisela (Koch, 1999)
TABLE CYP-23. Hyponex medium for seed germination of
Cypripedium macranthos
var.
rebunense
(Shimura and Koda, 2004)
TABLE CYP-24. Hyponex medium for seed germination of
Cypripedium macranthos
var.
rebunense
(Shimura and Koda, 2004)
TABLE CYP-25. Hyponex medium for callus induction from protocorms of
Cypripedium macranthos
var.
rebunense
(Shimura and Koda, 2004)
TABLE CYP-26. Hyponex medium for plantlet formation from PLBs of
Cypripedium macranthos
var.
rebunense
(Shimura and Koda, 2004)
TABLE CYP-27. Quarter-strength Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of
Cypripedium formosanum
explants (Lee, 2010)
TABLE CYP-28. Quarter-strength Murashige–Skoog medium (Murashige and Skoog, 1962) modified for root induction on
Cypripedium formosanum
shoots (Lee, 2010)
TABLE CYRT-1. Vacin and Went medium (Vacin and Went, 1949) modified for the culture of
Cyrtopodium
root tips, formation of protocorm-like bodies, and plantlet regeneration (Sanchez, 1988)
TABLE CYRT-2. Effects of auxin, cytokinin, and coconut water on
Cyrtopodium
root tips in vitro (Sanchez, 1988)
TABLE DAR-1. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of axillary buds of
Darwinara
(Kishi and Takagi, 1997)
TABLE DAR-2. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for plantlet production from protocorm-like bodies of
Darwinara
(Kishi and Takagi, 1997)
TABLE DEN-1. Characteristics and success of cultured
Dendrobium
buds (Kim et al., 1970)
TABLE DEN-2. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
shoot tips (Sagawa et al., 1966)
TABLE DEN-3. Media requirements of
Dendrobium
species and hybrids (Lim-Ho, 1981)
TABLE DEN-4. Modified Vacin and Went medium (Vacin and Went, 1949) for
Dendrobium
bud cultures (Lim-Ho, 1981)
TABLE DEN-5. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
buds (Lim-Ho, 1981)
TABLE DEN-6. Modified Knudson C medium (Knudson, 1946) for the culture of
Dendrobium
buds (Lim-Ho, 1981)
TABLE DEN-7. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for shoot production by
Dendrobium
seedling-tip explants (Gandawijaja, 1980)
TABLE DEN-8. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for root induction on shoots produced by
Dendrobium
seedling-tip explants (Gandawijaja, 1980)
TABLE DEN-9. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
shoot tips (Fernando, 1979)
TABLE DEN-10. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
buds (H. Singh, 1976)
TABLE DEN-11. Modified Vacin and Went medium (Vacin and Went, 1949) for the proliferation of protocorm-like bodies from
Dendrobium
buds (H. Singh, 1976)
TABLE DEN-12. Modified Vacin and Went medium (Vacin and Went, 1949) for plantlet formation from protocorm-like bodies of
Dendrobium
(H. Singh, 1976)
TABLE DEN-13. Modified Reinert–Mohr medium (Reinert and Mohr, 1967) for shoot induction from explants of
Dendrobium antennatum
(Kukułczanka and Wojciechowska, 1983)
TABLE DEN-14. Modified Reinert–Mohr medium (Reinert and Mohr, 1967) for shoot induction from explants of
Dendrobium phalaenopsis
(Kukułczanka and Wojciechowska, 1983)
TABLE DEN-15. Modified Reinert–Mohr medium (Reinert and Mohr, 1967) for root induction on shoots of
Dendrobium antennatum
and
Dendrobium phalaenopsis
(Kukułczanka and Wojciechowska, 1983)
TABLE DEN-16. Modified Reinert–Mohr medium (Reinert and Mohr, 1967) for callus induction from explants of
Dendrobium phalaenopsis
(Kukułczanka and Wojciechowska, 1983)
TABLE DEN-17. Modified Vacin and Went medium (Vacin and Went, 1949) for initial culture of shoot tips of
Dendrobium
Jaquelyn Thomas ‘White’ (Soediono, 1983
b
)
TABLE DEN-18. Modified Vacin and Went medium (Vacin and Went, 1949) for initial culture of shoot tips of
Dendrobium
Jaquelyn Thomas ‘White’ (Soediono, 1983
b
)
TABLE DEN-19. Modified Knop’s medium for the culture of
Dendrobium
node sections (Mosich et al., 1973, 1974
a
, 1974
b
)
TABLE DEN-20. Modified Murashige–Skoog medium for root induction on
Dendrobium
shoots obtained from node sections (Mosich et al., 1974
a
)
TABLE DEN-21. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
and
Phalaenopisis
flower-stalk cuttings (Intuwong et al., 1972
a
, 1972
b
)
TABLE DEN-22. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of seedling leaves of
Dendrobium crumenatum
(Manorama et al., 1986)
TABLE DEN-23. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for the culture of leaves from mature plants of
Dendrobium crumenatum
(Manorama et al., 1986)
TABLE DEN-24. Modified Vacin and Went medium (Vacin and Went, 1949) for the culture of
Dendrobium
leaf bases (Fu, 1978, 1979
b
)
TABLE DEN-25. Knop’s medium (Knop, 1884) modified for the culture of
Dendrobium
stem nodes (Yam, 1989)
TABLE DEN-26. Murashige–Skoog medium (Murashige and Skoog, 1962) modified for the culture of pseudobulb segments of
Dendrobium chrysanthum
(Vij and Pathak, 1989)
TABLE DEN-27. Modified Murashige–Skoog medium (Murashige and Skoog, 1962) for callus and bud induction on pseudobulb segments of
Dendrobium chrysanthum
(Vij and Pathak, 1989)
TABLE DEN-28. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the production of protocorm-like bodies (PLBs) from
Dendrobium
shoot-tip explants (Sharon and Vasundhara, 1990)
TABLE DEN-29. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the production of plantlets from protocorm-like bodies (PLBs) derived from
Dendrobium
shoot-tip explants (Sharon and Vasundhara, 1990)
TABLE DEN-30. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the production of plantlets from protocorm-like bodies (PLBs) derived from
Dendrobium
shoot-tip explants (Sharon and Vasundhara, 1990)
TABLE DEN-31. Knudson C (KC) medium (Knudson, 1946) as modified for protocorm-like body (PLB) production from stem disk sections of
Dendrobium moschatum
(Kanjilal et al., 1999)
TABLE DEN-32. Knudson C (KC) medium (Knudson, 1946) as modified for protocorm-like bodies (PLBs) and plantlet development of
Dendrobium moschatum
(Kanjilal et al., 1999)
TABLE DEN-33. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of shoot explants of
Dendrobium moschatum
and
Dendrobium aphyllum
(Nayak et al., 1997
b
)
TABLE DEN-34. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of thin cross-section explants of
Dendrobium nobile
(Nayak et al., 2002)
TABLE DEN-35. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the culture of shoot-tip explants of
Dendrobium
Queen Sonia (Aswath, 2001)
TABLE DEN-36. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the proliferation of protocorm-like bodies (PLBs) of
Dendrobium
Queen Sonia (Aswath, 2001)
TABLE DEN-37. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for plantlet production from protocorm-like bodies (PLBs) of
Dendrobium
Queen Sonia (Aswath, 2001)
TABLE DEN-38. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of shoot-tip explants of
Dendrobium
Sonia (Prasad et al., 2001)
TABLE DEN-39. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the culture of
Dendrobium
Sonia shoots (Prasad et al., 2001)
TABLE DEN-40. Effects of sucrose and CO
2
levels on the growth of
Dendrobium
plantlets in vitro (Mitra et al., 1998)
TABLE DEN-41. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the culture of
Dendrobium
shoots (Mitra et al., 1998)
TABLE DEN-42. Vacin and Went (VW) medium (Vacin and Went, 1949) as used for the culture of
Dendrobium
tissues in vitro (Hew and Mah, 1989)
TABLE DEN-43. Vacin and Went (VW) medium (Vacin and Went, 1949) as modified for the differentiation
Dendrobium
tissues (Hew and Mah, 1989)
TABLE DEN-44. Media formulations as presented by Oyamada and Takano (1990) in their Table 1
TABLE DEN-45. Oyamada and Takano (OT) medium (Oyamada and Takano, 1990) for the culture of basal buds of
Dendrobium
Formidible (Oyamada and Takano, 1990)
TABLE DEN-46. Oyamada and Takano (OT) medium (Oyamada and Takano, 1990) for the proliferation of PLBs of
Dendrobium
Formidible (Oyamada and Takano, 1990)
TABLE DEN-47. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the proliferation of
Dendrobium candidum
protocorms (G.Y. Wang et al., 1993)
TABLE DEN-48. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the preculture of
Dendrobium candidum
protocorms for in vitro flowering (G.Y. Wang et al., 1993)
TABLE DEN-49. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for flower induction in vitro in
Dendrobium candidum
(G.Y. Wang et al., 1993)
TABLE DEN-50. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Dendrobium
protoplasts (Kuehnle and Nan, 1990)
TABLE DEN-51. Inorganic salts of the Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) and the organic constituents of the Kao and Michayluk (KM) medium (Kao and Michayluk, 1975) for the culture of
Dendrobium
protoplasts (Yasugi, 1990)
TABLE DEN-52. Diluted inorganic salts of the Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) and the organic constituents of the Kao and Michayluk (KM) medium (Kao and Michayluk, 1975) for the culture of
Dendrobium
protoplasts (Yasugi, 1990)
TABLE DEN-53. Liquid Vacin and Went (VW) medium (Vacin and Went, 1949) modified for proliferation of
Cymbidium
mosaic virus (CyMV)-infected protocorm-like bodies (PLBs) of
Dendrobium
Jaquelyn Thomas ‘Uniwai Mist’ (Porter and Kuehnle, 1997)
TABLE DEN-54. Liquid ribavirin-containing Vacin and Went (VW) medium (Vacin and Went, 1949) modified for eradication of
Cymbidium
mosaic virus (CyMV) from protocorm-like bodies (PLBs) of
Dendrobium
Jaquelyn Thomas ‘Uniwai Mist’ (Porter and Kuehnle, 1997)
TABLE DEN-55. Solid ribavirin-containing Vacin and Went (VW) medium (Vacin and Went, 1949) modified for eradication of
Cymbidium
mosaic virus (CyMV) from protocorm-like bodies (PLBs) and plantlets of
Dendrobium
Jaquelyn Thomas ‘Uniwai Mist’ (Porter and Kuehnle, 1997)
TABLE DEN-56. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of
Dendrobium wardianum
shoot tips (Sharma et al., 1992)
TABLE DEN-57. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for culture and proliferation of synthetic seeds of
Dendrobium wardianum
(Sharma et al., 1992)
TABLE DEN-58. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for plantlet production from synthetic seeds of
Dendrobium wardianum
(Sharma et al., 1992)
TABLE DEN-59. Sodium alginate-containing Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for production of synthetic seeds of
Dendrobium wardianum
(Sharma et al., 1992)
TABLE DEN-60. Calcium chloride-containing Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for preparation of synthetic seeds of
Dendrobium wardianum
(Sharma et al., 1992)
TABLE DEN-61. Mitra, Prasad, and Roychowdhury (MPR) medium (Mitra et al., 1976) modified for proliferation of
Dendrobium densiflorum
protocorm-like bodies (PLBs) derived from node cultures (Vij et al., 2001)
TABLE DEN-62. Mitra, Prasad, and Roychowdhury (MPR) medium (Mitra et al., 1976) for maintenance of protocorm-like bodies (PLBs) of
Dendrobium densiflorum
(Vij et al., 2001)
TABLE DEN-63. Sodium alginate-containing Mitra, Prasad, and Roychowdhury (MPR) medium (Mitra et al., 1976) for encapsulation of protocorm-like bodies (PLBs) of
Dendrobium densiflorum
(Vij et al., 2001)
TABLE DEN-64. Calcium chloride solution for the encapsulation of protocorm-like bodies (PLBs) of
Dendrobium densiflorum
(Vij et al., 2001)
TABLE DEN-65. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for flower induction in
Dendrobium moniliforme
seedlings (Duan and Yazawa, 1994
b
)
TABLE DEN-66. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of shoot tips of
Dendrobium wardianum
(Sharma and Tandon, 1991)
TABLE DEN-67. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of plantlets produced from protocorm-like bodies (PLBs) derived from shoot tips of
Dendrobium wardianum
(Sharma and Tandon, 1991)
TABLE DEN-68. B5 medium (Gamborg et al., 1968) as modified for the strengthening of
Dendrobium moniliforme
plantlets (Liu and Zhang, 1989)
TABLE DEN-69. Half-strength Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) as modified for the strengthening of
Dendrobium moniliforme
plantlets (Liu and Zhang, 1989)
TABLE DEN-70. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of shoot-tip and bud explants of
Dendrobium
Earsakul (Kim et al., 2003)
TABLE DEN-71. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of shoot-tip and bud explants of
Dendrobium
Omya ‘Pink’ (Kim et al., 2003)
TABLE DEN-72. Vacin and Went (VW) medium (Vacin and Went, 1949) modified for the culture of shoot-tip and bud explants of
Dendrobium
Omya ‘Pink’ (Kim et al., 2003)
TABLE DEN-73. Basal Vacin and Went (VW) medium (Vacin and Went, 1949)
TABLE DEN-74. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of shoot tips of
Dendrobium
Sonia (Saiprasad and Polisetty, 2003)
TABLE DEN-75. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the culture of sections of shoot-tip-derived protocorm-like bodies (PLBs) of
Dendrobium
Sonia (Saiprasad and Polisetty, 2003)
TABLE DEN-76. Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) modified for the germination of encapsulated shoot-tip-derived protocorm-like bodies (PLBs) of
Dendrobium
Sonia (Saiprasad and Polisetty, 2003)
TABLE DEN-77. Sodium alginate-containing Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for encapsulation of shoot-tip-derived protocorm-like bodies (PLBs) of
Dendrobium
Sonia (Saiprasad and Polisetty, 2003)
TABLE DEN-78. Calcium chloride solution for encapsulation of shoot-tip-derived protocorm-like bodies (PLBs) of
Dendrobium
Sonia (Saiprasad and Polisetty, 2003)
TABLE DEN-79. Putrescine-containing Murashige–Skoog (MS) medium (Murashige and Skoog, 1962) for proliferation of protocorm-like bodies (PLBs) of
Dendrobium
Sonia (Saiprasad et al., 2004)
TABLE DEN-80. Knudson C (KC) medium (Knudson, 1946) modified for the culture of
Dendrobium fimbriatum
var.
oculatum
shoot-tip explants (Roy and Banerjee, 2003)
TABLE DEN-81. Knudson C (KC) medium (Knudson, 1946) modified for proliferation of shoot-tip-derived callus of
Dendrobium fimbriatum
var.
oculatum
, production of protocorm-like bodies (PLBs), and plantlet regeneration (Roy and Banerjee, 2003)
TABLE DEN-82. Half-strength Murashige–Skoog (½MS) medium (Murashige and Skoog, 1962) for the germination of
Dendrobium candidum
seeds (Shiau et al., 2005)
TABLE DEN-83. Half-strength Murashige–Skoog (½MS) medium (Murashige and Skoog, 1962) for the culture of
Dendrobium candidum
seedlings (Shiau et al., 2005)
TABLE DEN-84. Half-strength Murashige–Skoog (½MS) medium (Murashige and Skoog, 1962) for the culture of node explants from seedlings of
Dendrobium candidum
(Shiau et al., 2005)
TABLE DEN-85. Half-strength Murashige–Skoog (½MS) medium (Murashige and Skoog, 1962) for the rooting of
Dendrobium candidum
shoots (Shiau et al., 2005)
TABLE DEN-86. Modified Knudson C (mKC) medium (Knudson, 1946) for the germination of
Dendrobium chrysotoxum
seeds (Roy et al., 2007)
TABLE DEN-87. Modified Knudson C (mKC) medium (Knudson, 1946) for the culture of explants from
Dendrobium chrysotoxum
seedlings (Roy et al., 2007)
TABLE DEN-88. Modified Knudson C (mKC) medium (Knudson, 1946) for the induction of protocorm-like bodies (PLBs) on explants of
Dendrobium chrysotoxum
seedlings (Roy et al., 2007)
TABLE DEN-89. Hormone-containing modified Knudson C (mKCdfo) medium (Knudson, 1946) for the culture of
Dendrobium fimbriatum
var.
oculatum
(Roy and Banerjee, 2003)
TABLE DEN-90. Hormone-free modified Knudson C (mKCdfo) medium (Knudson, 1946) for the culture of
Dendrobium fimbriatum
var.
oculatum
(Roy and Banerjee, 2003)
TABLE DEN-91. Kinetin-containing half-strength Murashige–Skoog (hMS) medium (Murashige and Skoog, 1962) for the culture of
Dendrobium
Sonia flower-stalk nodes (Martin et al., 2006)
TABLE DEN-92. Coconut water-containing half-strength Murashige–Skoog (hMS) medium (Murashige and Skoog, 1962) for the culture of
Dendrobium
Sonia flower-stalk nodes (Martin et al., 2006)
TABLE DEN-93. Activated charcoal (AC)-containing half-strength Murashige–Skoog (hMS) medium (Murashige and Skoog, 1962) for root induction on shoots of
Dendrobium
Sonia (Martin et al., 2006)
