Progress in Food Preservation E-Book

• Herausgeber: John Wiley & Sons
• Kategorie: Fachliteratur
• Sprache: Englisch

Seitenzahl: 1440

Veröffentlichungsjahr: 2012

Beschreibung

This volume presents a wide range of new approaches aimed at improving the safety and quality of food products and agricultural commodities. Each chapter provides in-depth information on new and emerging food preservation techniques including those relating to decontamination, drying and dehydration, packaging innovations and the use of botanicals as natural preservatives for fresh animal and plant products. The 28 chapters, contributed by an international team of experienced researchers, are presented in five sections, covering: * Novel decontamination techniques * Novel preservation techniques * Active and atmospheric packaging * Food packaging * Mathematical modelling of food preservation processes * Natural preservatives This title will be of great interest to food scientists and engineers based in food manufacturing and in research establishments. It will also be useful to advanced students of food science and technology.

Leseprobe

Contents

Cover

Title Page

Copyright

Preface

Contributors

Part I: Active and Atmospheric Packaging

Chapter 1: Selected Techniques to Decontaminate Minimally Processed Vegetables

1.1 Introduction

1.2 UV-C Light

1.3 Pulsed Light

1.4 Electrolysed Oxidizing Water

1.5 Ozone

1.6 Low-Temperature Blanching

References

Chapter 2: Active and Intelligent Packaging of Food

2.1 Introduction

2.2 Active Scavengers

2.3 Active Releasers/Emitters

2.4 Intelligent Packaging

2.5 Nanotechnology in Active and Intelligent Packaging

2.6 Future Trends

2.7 Further Sources of Information

References

Chapter 3: Modified-Atmosphere Storage of Foods

3.1 Introduction

3.2 Modified Atmosphere

3.3 Effects of Modified Gas Atmospheres on Microorganisms and Foods

3.4 Application of Modified Atmospheres for Food Preservation

3.5 Food Safety and Future Outlook

3.6 Conclusions

References

Chapter 4: Effects of Combined Treatments with Modified-Atmosphere Packaging on Shelf-Life Improvement of Food Products

4.1 Introduction

4.2 Physical Treatments

4.3 Chemical Treatments

4.4 Quality-Improving Agents

4.5 Antibrowning Agents

4.6 Natural Products

4.7 Other Methods, Such as Oxygen Scavengers and Coatings

4.8 Biocontrol

References

Chapter 5: Coating Technology for Food Preservation

5.1 Introduction

5.2 Progress in Relevant Materials and their Applications in Coating

5.3 Progress in Coating Methodology

5.4 Future Trends in Coating Technology

5.5 Conclusions

References

Part II: Novel Decontamination Techniques

Chapter 6: Biological Materials and Food-Drying Innovations

6.1 Introduction

6.2 Microwave Drying

6.3 Radio Frequency Drying

6.4 Infrared Drying

6.5 Refractance Window™ Drying

References

Chapter 7: Atmospheric Freeze Drying

7.1 Introduction

7.2 Basic Principles

7.3 Types of Atmospheric Freeze Dryer and Application

7.4 A Novel Approach to AFD

7.5 Model

7.6 Conclusions

References

Chapter 8: Osmotic Dehydration: Theory, Methodologies, and Applications in Fish, Seafood, and Meat Products

8.1 Introduction

8.2 Methods of Drying

8.3 Some Results

8.4 Conclusions

References

Chapter 9: Dehydration of Fruit and Vegetables in Tropical Regions

9.1 Introduction

9.2 Forms of Water

9.3 Advantages of Dried Foods

9.4 Drying Processes

9.5 Dehydration

9.6 Evaporation and Concentration

9.7 Spoilage of Dried Fruits and Vegetables

9.8 Merits of Dehydration over Sun Drying

9.9 Effects of Dehydration on Nutritive Value of Fruits and Vegetables

9.10 Effects of Drying on Microorganisms

9.11 Effect of Drying on Enzyme Activity

9.12 Influence of Drying on Pigments

9.13 Reconstitution Test

9.14 Drying Parameters

References

Chapter 10: Developments in the Thermal Processing of Food

10.1 Introduction

10.2 Thermal Processing

10.3 Innovative Thermal Processing Techniques

References

Chapter 11: Ozone in Food Preservation

11.1 Introduction

11.2 History

11.3 Chemistry

11.4 Generation

11.5 Antimicrobial Effect

11.6 Applications

11.7 Toxicity and Safety of Personnel

11.8 Conclusion

References

Chapter 12: Application of High Hydrostatic Pressure Technology for Processing and Preservation of Foods

12.1 Introduction

12.2 The Working Principles of HIGH HYDROSTATIC PRESSURE

12.3 Microbial Inactivation by HIGH HYDROSTATIC PRESSURE

12.4 Effect of High Pressure on the Physical and Biochemical Characteristics of Food Systems

12.5 Applications of HIGH HYDROSTATIC PRESSURE to Specific Food Commodities

12.6 CONCLUSIONS

References

Chapter 13: Pulsed Electric Fields for Food Preservation: An Update on Technological Progress

13.1 Introduction

13.2 Historical Background of PULSED ELECTRIC FIELDS

13.3 PULSED ELECTRIC FIELD Processing

13.4 Mechanisms and Factors Affecting Pulsed Electric Fields

13.5 pulsed electric field Applications in Food Processing

13.6 Nanosecond pulsed electric fields

13.7 Impacts of pulsed electric fields on Antioxidant Features

13.8 Effects of pulsed electric fields on Solid Textures

13.9 Starch Modification by pulsed electric fields

13.10 Conclusions

References

Chapter 14: Salting Technology in Fish Processing

14.1 Introduction

14.2 Process Steps in Salting Technology

14.3 Factors Affecting the Penetration of Salt

14.4 Ripening of Salted Fish

14.5 Conclusion

References

Chapter 15: Hypoxanthine Levels, Chemical Studies and Bacterial Flora of Alternate Frozen/Thawed Market-Simulated Marine Fish Species

15.1 Introduction

15.2 Sources of Contamination of Fish

15.3 Fish as a Perishable Food

15.4 Indicators of Deterioration in Frozen Fish

15.5 Bacterial Food Poisoning in Seafood

15.6 Methods Used for Assessing Deteriorative Changes in Fish

15.7 Study of Three Marine Fish Species

15.8 Conclusions

Dedication

References

Chapter 16: Preservation of Cassava (Manihot esculenta Crantz): A Major Crop to Nourish People Worldwide

16.1 Introduction: Cassava Production and Importance

16.2 Nutritional Value

16.3 Cassava Utilization

16.4 Factors that Limit Cassava Utilization, and its Toxicity

16.5 Cassava Processing

16.6 Storage of Processed Cassava Products

References

Chapter 17: Use of Electron Beams in Food Preservation

17.1 Introduction

17.2 Food Irradiation, Source and Technology

17.3 THE Food Industry and Electron-Beam Irradiation

17.4 Electron-Beam Irradiation And Microorganisms

17.5 Conclusion and Future Outlook

References

Part III: Modelling

Chapter 18: Treatment of Foods using High Hydrostatic Pressure

18.1 Introduction

18.2 Pressure and the Earth

18.3 Main Factors Characterizing High Hydrostatic Pressure

18.4 Historical Perspective

18.5 HIGH HYDROSTATIC PRESSURE Process and Equipment

18.6 Commercial High Hydrostatic Pressure-Treated Food Products Around the World

18.7 Consumer Acceptance of High Hydrostatic Pressure Processing

References

Chapter 19: Role of Predictive Microbiology in Food Preservation

19.1 Microorganisms in Foods

19.2 Predictive Microbiology

19.3 Software Packages and Web Applications in Predictive Microbiology

19.4 Applications of Predictive Microbiology in Food Preservation

References

Chapter 20: Factors Affecting the Growth of Microorganisms in Food

20.1 Introduction

20.2 Intrinsic Factors

20.3 Extrinsic Factors

20.4 Implicit Factors

20.5 Processing Factors

20.6 Interaction between Factors

References

Chapter 21: A Whole-Chain Approach to Food Safety Management and Quality Assurance of Fresh Produce

21.1 Introduction: The Management of Food Safety Requires a Holistic Approach

21.2 Microbial Quality Management Starts in Production

21.3 Processing of Fresh Produce is a Key Step in Quality Preservation

21.4 Monitoring the Entire Food Supply Chain

21.5 The Improvement of Compliance by Increasing Awareness

21.6 Last but Not Least: Consumers

Conclusion

Acknowledgements

References

Part IV: Use of Natural Preservatives

Chapter 22: Food Bioprotection: Lactic Acid Bacteria as Natural Preservatives

22.1 Introduction

22.2 Antimicrobial Potential of Lab

22.3 Bacteriocins

22.4 Food Applications

22.5 Hurdle Technology to Enhance Food Safety

22.6 Bacteriocins in Packaging Films

22.7 Conclusions

References

Chapter 23: Bacteriocins: Recent Advances and Opportunities

23.1 Introduction

23.2 Bacteriocins Produced BY Lab

23.3 Bioprotection Against Pathogenic Bacteria

23.4 Bioprotection Against Spoilage Microorganisms

23.5 Medical and Veterinary Potential of Lab Bacteriocins

23.6 Conclusion

Acknowledgements

References

Chapter 24: Application of Botanicals as Natural Preservatives in Food

24.1 Introduction

24.2 Antibacterials

24.3 Antifungals

24.4 Antioxidants

24.5 Applications

24.6 Conclusion

References

Chapter 25: Tropical Medicinal Plants in Food Processing and Preservation: Potentials and Challenges

25.1 Introduction

25.2 Some Tropical Medicinal Plants with Potential Food-Processing Value

25.3 Conclusion

References

Chapter 26: Essential Oils and Other Plant Extracts as Food Preservatives

26.1 Background

26.2 Secondary Metabolites of Plants

26.3 Modes of Action of Essential Oils and Plant Extracts

26.4 Specific Applications of Plant Extracts in the Food Industry

26.5 Medicinal Plants and the Regulations Governing the use of Botanical Biocides

26.6 Future Perspectives

26.7 Conclusions

References

Chapter 27: Plant-Based Products as Control Agents of Stored-Product Insect Pests in the Tropics

27.1 Introduction

27.2 Common Insect Pests of Stored Food Grains in the Tropics

27.3 Advances in Stored-Product Insect Pest Control in the Tropics

27.4 Advances in Development of Botanical Pesticides in the Tropics

27.5 Prospects of Botanical Pesticides

References

Chapter 28: Preservation of Plant and Animal Foods: An Overview

28.1 Introduction: Definition and Principles

28.2 Food Preservation Methods

28.3 Conclusion

References

Index

This edition first published 2012 © 2012 by John Wiley & Sons Ltd.

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Library of Congress Cataloging-in-Publication Data

Progress in food preservation / edited by Rajeev Bhat, Abd Karim Alias, Gopinadhan Paliyath.

p. cm.

Includes bibliographical references and index.

ISBN-13: 978-0-470-65585-6 (hard cover : alk. paper)

ISBN-10: 0-470-65585-2

1. Food–Preservation–Research. I. Bhat, Rajeev. II. Alias, Abd Karim. III. Paliyath, Gopinadhan.

TP371.2.P76 2012

664'.028–dc23

2011024817

A catalogue record for this book is available from the British Library.

Wiley also publishes its books in a variety of electronic formats. Some content that appears in print may not be available in electronic books.

Preface

Food preservation is a critical control point that influences and determines a whole range of outcomes, ranging from preservation of nutritional quality, food safety, the wholesome nature of food, texture, taste and organoleptic qualities, and consumer appeal, along with compliance to several points in the value chain that include long-term storage, long-distance transportation and marketing. In an era that is becoming increasingly global, the economics of food preservation, shipping and transportation determine not only the availability of food globally, but also the availability of food to the consumer at a reasonable price that can sustain the whole food value chain. This is especially critical in situations involving the shipping of fresh food over large distances. If the perishability is high, the food must reach the destination in a short time to enhance the market window.

Geopolitical and climatic turmoil in recent times have increased the cost of fuel, resulting in increased food prices across the globe. Food prices in Canada were anticipated to increase over 5% in 2011, which is a substantial increase in a country that has enjoyed relatively stable prices for food. The price of fresh vegetables in India rose by over 100% in 2011. Such events highlight the need for food preservation practices that will enable the buffering of worldwide fluctuations in food prices, while enhancing food safety and dealing with security issues across the world (see the UN Secretary General's High-Level Task Force on the Global Food Security Crisis, www.un.org/issues/food/taskforce/index.shtml).

There are several factors that influence the properties of preserved food, and these factors determine the nature and method of preservation techniques that are employed. Preservation of dry foods with low water activity is relatively easy. When it comes to highly perishable foods such as meat, seafoods, fruits and vegetables, this is a challenge. In animal products, the major stress is on the prevention of microbial growth, and preservation techniques are used to achieve this goal. The shelf life of fruits and vegetables is highly variable. Rapidly respiring commodities have a very short shelf life, and consequently methods involving low temperature and anaerobic conditions are favoured. This is especially true for fresh-cut fruits and vegetables. Sun drying or drying in general has been practised as a mode of food preservation for centuries. Thus, reducing water activity is an efficient method for food preservation. The application of concentrated osmotic solutions for dehydration is another way to achieve the same goal. In every method, there is an added element of food safety and killing harmful microorganisms is essential to ensure the preservation as well as the safety of food. Food preservation methods involving thermal and non-thermal techniques have been widely employed in the food industry. At present, there are several new methods concurrently used in conjunction with traditional methods, making use of such technologies as microwaves, electricity (pulsed field), high pressure and irradiation, to provide better preservation of food.

This book has been compiled to provide an in-depth evaluation of the recent advances in the science and technology dealing with food preservation. The chapters of the book are organized into four parts. The first part contains five chapters discussing various aspects of modified-atmosphere packaging, active packaging and coating technology. The second part deals with novel decontamination techniques, describing several modern trends in this area. The third section comprises four chapters which evaluate aspects of theoretical modelling in relation to various aspects of food preservation. The fourth part deals with the use of natural preservatives or methods for food preservation. Altogether, the chapters are written by experts in their respective fields and provide a complete approach to food preservation technologies, as applied to various food systems.

The technical help rendered in various ways by David McDade, Andrew Harrison, Nik Prowse, Alison Nick (Wiley-Blackwell) and Mrs Ranjana Hegde (Malaysia) is gratefully acknowledged. We, the editors, thank all the authors for their immense support and for their vital contributions.

Rajeev Bhat, Abd Karim Alias and Gopinadhan Paliyath

Contributors

Gabriel O. AdegokeDepartment of Food TechnologyFaculty of TechnologyUniversity of IbadanIbadan, Nigeria

Hami AlpasFood Engineering DepartmentMiddle East Technical UniversityAnkara, Turkey

Francisco Noé Arroyo-LópezCavanilles Institute for Biodiversity &Evolutionary BiologyUniversity of ValenciaEdifici d'Institus del Campus de PaternaValencia, Spain

Ioannis S. ArvanitoyannisDepartment of Agriculture, Icthyology and Aquatic EnvironmentUniversity of ThessalyNea Ionia Magnessias, Greece

Javaid Aziz AwanNational Institute of Food Science andTechnologyUniversity of AgricultureFaisalabad, Pakistan

Joaquí–n Bautista-GallegoDepartamento de Biotecnología deAlimentosInstituto de la Grasa (CSIC)Seville, Spain

Philip K. BettDepartment of Biological SciencesEgerton UniversityEgerton, Kenya

Rajeev BhatFood Technology DivisionSchool of Industrial TechnologyUniversiti Sains MalaysiaPenang, Malaysia

Rhoda J. BirechDepartment of Crops, Horticultureand SoilsEgerton UniversityEgerton, Kenya

Leon BrimerDepartment of Veterinary Disease BiologyFaculty of Life SciencesUniversity of CopenhagenFrederiksberg C, Denmark

Sencer BuzrulTütün ve Alkol Piyasası DüzenlemeKurumu (TAPDK)Ankara, Turkey

Nawel ChaftarFaculté de Médecine Ibn El JazzarDépartement de BiochimieSousse, Tunisia

Chamorn ChawengkijwanichCentral Research UnitNational Nanotechnology CenterNational Science and TechnologyDevelopment AgencyKlong LuangPathumthani, Thailand

Haiqiang ChenDepartment of Animal and Food SciencesUniversity of DelawareNewark, DE, USA

Sandra CombrinckDepartment of ChemistryTshwane University of TechnologyPretoria, South Africa

Raf De VisiMIK, the Institute for MicrobialControl of the Food ChainSint-Katelijne-Waver, BelgiumResearch Centre for Vegetable ProductionSint-Katelijne-Waver, Belgium

Arop L. DengDepartment of Biological SciencesEgerton UniversityEgerton, Kenya

Wilma Du PlooyJohn Bean TechnologiesSouth Africa (Pty) LtdBrackenfell, South Africa

Afolabi F. EleyinmiHuman Resources and SkillsDevelopment Canada(Service Canada)Edmonton, AB, Canada

Osman ErkmenDepartment of Food EngineeringFaculty of EngineeringUniversity of GaziantepGaziantep, Turkey

brahim ErkoyuncuDepartment of Fishing andProcessing TechnologyFisheries FacultyUniversity of SinopSinop, Turkey

Antonio Garrido-FernándezDepartamento de Biotecnología deAlimentosInstituto de la Grasa (CSIC)Seville, Spain

Taoufik GhrairiFaculté de Médecine Ibn El JazzarDépartement de BiochimieSousse, Tunisia

G.J. Benoit GnonlonfinBiosciences Eastern and Central AfricaInternational Livestock Research InstituteNairobi, Kenya

Vicente M. Gómez-LópezInstituto de Ciencia y Tecnología deAlimentosFacultad de CienciasUniversidad Central de VenezuelaCaracas, Venezuela

Vibha GuptaDepartment of BotanyGuru Nanak Khalsa CollegeMatunga, Mumbai, India

Siddig Hussein HamadDepartment of Food and Nutrition SciencesCollege of Agricultural and Food SciencesKing Faisal UniversitySaudi Arabia

Khaled HaniFaculté de Médecine Ibn El JazzarDépartement de BiochimieSousse, Tunisia

Inge HansseniMIK, the Institute for Microbial Control ofthe Food ChainSint-Katelijne-Waver, Belgiumand Scientia Terrae Research InstituteSint-Katelijne-Waver, Belgium

Abd Karim AliasFood Technology DivisionSchool of Industrial TechnologyUniversiti Sains MalaysiaPenang, Malaysia

Habib KocabıyıkDepartment of Agricultural MachineryAgricultural FacultyCanakkale Onsekiz Mart UniversityCanakkale, Turkey

Phikunthong KopermsubCentral Research UnitNational Nanotechnology CenterNational Science and TechnologyDevelopment AgencyKlong LuangPathumthani, Thailand

Matthew S. KrauseiMIK, the Institute for Microbial Control ofthe Food ChainSint-Katelijne-Waver, Belgium

Shengmin LuInstitute of Food ProcessingZhejiang Agricultural SciencesHangzhou, People's Republic of China

Rita MoloneyEnvironmental Health ServiceHealth Service ExecutiveEnnis, Co. Clare, Ireland

Arun S. MujumdarMinerals, Metals and MaterialsTechnology Center (M3TC)and Department of MechanicalEngineeringNational University of SingaporeSingapore

Abdorreza Mohammadi NafchiFood Science andTechnology DivisionDepartment of Agriculture ScienceIslamic Azad UniversityDamghan Branch, DamghanSemnan, Iran

Jagdish NairDepartment of Biotechnology andBioinformaticsPadmashree Dr. D. Y.Patil UniversityNavi Mumbai, India

Hudaa NeetooDepartment of Animal andFood SciencesUniversity of DelawareNewark, DE, USA

Joshua O. OgendoDepartment of Crops,Horticulture and SoilsEgerton UniversityEgerton, Kenya

Abiodun A. OlapadeDepartment of Food TechnologyFaculty of TechnologyUniversity of IbadanIbadan, Nigeria

Tareq M. OsailiDepartment of Nutrition and FoodTechnologyFaculty of AgricultureJordan University of Science andTechnologyIrbid, Jordan

Olusegun A. OyeleseDepartment of Wildlife andFisheriesManagementUniversity of IbadanIbadan, Nigeria

Gopinadhan PaliyathDepartment of Plant AgricultureUniversity of GuelphGuelph, Canada

Panagiota PanagiotakiDepartment of Agriculture,Icthyology andAquatic EnvironmentUniversity of ThessalyNea Ionia Magnessias, Greece

Shek Mohammod Atiqure RahmanDepartment of Mechanical EngineeringMonash University (Sunway Campus)Selangor Darul Ehsan, Malaysia

Raúl RayaCentro de Referencia para Lactobacilos(CERELA)Consejo Nacional de InvestigacionesCientíficas y Técnicas (CONICET)Tucumán, Argentina

Hans RediersLaboratory for ProcessMicrobial Ecology andBio-Inspirational ManagementDepartment of Microbial andMolecular Systems (M2S)Katholieke Universiteit LeuvenAssociationSint-Katelijne-Waver, Belgium

Thierry RegnierDepartment of ChemistryTshwane University of TechnologyPretoria, South Africa

Salim-ur-RehmanNational Institute of Food Science andTechnologyUniversity of AgricultureFaisalabad, Pakistan

Lucila SaavedraCentro de Referencia para Lactobacilos(CERELA)Consejo Nacional de InvestigacionesCientíficas y Técnicas (CONICET)Tucumán, Argentina

Ambaliou SanniBiochemistry and Molecular BiologyLaboratoryFaculty of Sciences and TechniquesUniversity of Abomey-Calavi, BeninCotonou, Benin

Fernando SesmaCentro de Referencia para Lactobacilos(CERELA)Consejo Nacional de InvestigacionesCientíficas y Técnicas (CONICET)Tucumán, Argentina

István SiróFaculty of Food ScienceCorvinus University of BudapestBudapest, Hungary

Hülya TuranDepartment of Fishing and ProcessingTechnologyFisheries FacultyUniversity of SinopSinop, Turkey

Ado Van AsscheProcess Microbial Ecology and Bio-Inspirational Management Research GroupDepartment of Microbial and MolecularSystems (M2S)Katholieke Universiteit LeuvenAssociationSint-Katelijne-Waver, Belgium

Agapi VeikouDepartment of Agriculture, Icthyology andAquatic EnvironmentUniversity of ThessalyNea Ionia Magnessias, Greece

Graciela VignoloCentro de Referencia para Lactobacilos(CERELA)Consejo Nacional de InvestigacionesCientíficas y Técnicas (CONICET)Tucumán, Argentina

Kris A. WillemsiMIK, the Institute for MicrobialControl of the Food ChainSint-Katelijne-Waver, BelgiumProcess Microbial Ecology and Bio-Inspirational Management ResearchGroupDepartment of Microbial and MolecularSystems (M2S)Katholieke Universiteit LeuvenAssociationSint-Katelijne-Waver, Belgium

Qile XiaInstitute of Food ProcessingZhejiang Agricultural SciencesHangzhou, People's Republic of China

Bülent ZorlugençAgricultural FacultyFood Engineering Department andBiotechnology Research andApplication CentreÇukurova UniversityAdana, Turkey

Feyza Kırolu ZorlugençAgricultural FacultyFood Engineering DepartmentÇukurova UniversityAdana, Turkey

Part I

Active and Atmospheric Packaging

Chapter 1

Selected Techniques to Decontaminate Minimally Processed Vegetables

Vicente M. Gómez-López

Abstract: Production of minimally processed vegetables entails a big challenge. The product should be stable, fresh-looking and safe. Cutting and/or peeling accelerate physiological degradation and expose inner tissues to contamination, decreasing produce shelf life and increasing the risk of foodborne pathogen growth. Use of decontamination techniques to control microbial populations is limited because they must be mild enough to avoid impairing sensory characteristics of the product. As a consequence, novel decontamination techniques and decontaminating agents are being tested to cope with the challenge posed by this kind of product. This chapter addresses some novel developments and chemicals potentially useful to prolong the shelf life of minimally processed vegetables, namely continuous UV light, pulsed light, electrolysed oxidizing water, ozone and low-temperature blanching. It defines every technique or decontaminant, and then focuses on the effects on microbial population, produce physiology, sensory quality and nutritional consequences, if known.

Keywords: electrolysed oxidizing water; low-temperature blanching; minimal processing; novel decontamination agents; novel decontamination techniques; ozone; pulsed light; UV light

1.1 Introduction

During recent decades an intensive search for novel decontaminants and decontamination methods for minimally processed vegetables (MPV) has been pursued by researchers worldwide. The delicate nature of MPV and the absolute requirement of maintaining a fresh-looking product seriously limit the kind of techniques that can be used to obtain an innocuous and stable product.

A decontamination technique for MPV should reduce the risk of foodborne infections and intoxications, decrease microbial spoilage, preserve fresh attributes and nutritional quality, not leave hazardous residues or by-products (Gómez-López et al., 2009) and be environmentally friendly.

This chapter presents selected decontamination techniques that vary greatly, and which have not been extensively reviewed in recent years. It includes two photonic methods: UV-C light and pulsed light; a chemical method: ozonation; and a thermal method: low-temperature blanching. It briefly defines each method and its microbial inactivation mechanism, and then its effect on foodborne pathogenic and spoilage microorganisms, MPV shelf life and the sensory and nutritional quality of the MPV.

1.2 UV-C Light

1.2.1 Definition

UV-C is the portion of the electromagnetic spectrum corresponding to the band between 200 and 280 nm. Inactivation of microorganisms with UV systems can be performed by means of mercury lamps, xenon pulsed lamps, or excimer lasers. This section deals only with disinfection using mercury lamps, called continuous-wave UV (CW UV). UV inactivation of microorganisms is frequently achieved by using low-pressure mercury lamps designed to emit light at 254 nm, called germicidal light (Bintsis et al., 2000), or medium-pressure UV lamps that emit germicidal wavelengths from 200 to 300 nm (Bolton and Linden, 2003). Since UV light is a non-ionizing radiation, and irradiated products have had serious marketing problems, the term ‘illumination’ as proposed by Lagunas-Solar and Gómez-López (2007) will be used in place of ‘UV radiation’ or ‘UV irradiated’ to avoid misconception. UV-C illumination as a technique to preserve foods was discovered in the 1930s (Artés and Allende, 2005), and water disinfection by UV has been widely applied in Europe since the 1980s (Hijnen et al., 2006). In food-related industries, applications include disinfection of water supplies, food contact surfaces, air in food-preparation areas (Bintsis et al., 2000) and packaging materials (Mimouni, 2001).

UV illumination is characterized by being a relatively inexpensive and simple technique (although it is subject to certain safety precautions; Bintsis et al., 2000), the lack of residual compounds (López-Rubira et al., 2005) and the avoidance of chemicals that can cause ecological problems and/or are potentially harmful to humans (Allende and Artés, 2003b), although conventional mercury lamps generate ozone, which must be exhausted.

The technique is limited to decontaminating surfaces or transparent liquids due to the low penetrability of UV light. When illuminating a three-dimensional object, it is necessary to ensure that all surfaces receive adequate exposure to UV light, requiring equipment with radically new designs (Gardner and Shama, 2000).

The effect of UV light on microorganisms and foods depends on the energy incident on their surface, which is termed fluence (measured in Joules/meter2, J/m2).

1.2.2 Inactivation Mechanism

The germicidal effect of UV-C light is primarily due to the formation of thymine dimers (cyclobutane dimers) which inhibit the formation of new DNA chains (Mitchell et al., 1992; Giese and Darby, 2000). UV-C treatment of bacterial spores results mainly in formation of the ‘spore photoproduct’ 5-thyminyl-5,6-dihydrothymine (Slieman and Nicholson, 2000). Microbial cells can repair themselves from photochemical damage, mainly through photoreactivation, where the enzyme photolyase uses visible light energy to split cyclobutane dimers (Kao et al., 2005). Photoreactivation has been studied in water bodies; however, as far as is known by this author, its effect on decontaminated produce has not been studied.

1.2.3 Effect on Microbial Populations

The efficacy of UV-C to inactivate a wide range of microorganisms has been demonstrated in viruses (Eischeid et al., 2009), Gram-negative and Gram-positive bacteria (Chang et al., 1985; Yaun et al., 2003), bacterial spores (Mamane-Gravetz and Linden, 2004), conidia (Marquenie et al., 2002) and parasites (Zimmer et al., 2003; Hayes et al., 2008) in vitro as well as when inoculated onto (Yaun et al., 2004) and into vegetable matrixes (Hadjok et al., 2008).

UV-C illumination is able to increase vegetable shelf life based on microbial spoilage counts by either inactivating microorganisms and/or decreasing their growth rate, and can even trigger a lag phase. This lag phase is more apparent in yeasts than in other microbial populations (Erkan et al., 2001; Allende and Artés, 2003a, 2003b; Allende et al., 2006) and can account for lower counts in illuminated compared with control samples at the end of the storage time. In contrast, UV-C may sometimes stimulate growth of lactic acid bacteria, possibly due to a higher resistance to UV-C and elimination of competing microflora (Allende and Artés, 2003a, 2003b).

Erkan et al. (2001) treated tissue slices of zucchini squash with UV-C up to 9.86 kJ/m2, and Allende et al. (2006) did it to both sides of minimally processed (MP) Red Oak Leaf lettuce up to 7.11 kJ/m2. In these cases, UV-C did not inactivate total bacterial and yeast populations, but slowed down their growth during storage. In the latter example, this meant a shelf-life extension of at least 2 days. On the other hand, illumination of MP Red Oak Leaf lettuce (Allende and Artés, 2003a) and MP Lollo Rosso lettuce (Allende and Artés, 2003b) both with UV-C up to 8.14 kJ/m2 inactivated important microbial groups, bringing about a shelf-life prolongation of 2–3 days. In different cases, the published literature indicates that UV-C illumination of MPV can cause a shelf-life extension of at least 2 days from the microbial point of view when properly used.

1.2.4 Effect on Sensory Attributes

UV-C illumination has been reported to preserve or not affect the sensory quality of MPV. Erkan et al. (2001) stated that the decay of zucchini squash slices illuminated with UV-C at levels of 4.93 and 9.86 kJ/m2 was significantly less than controls during storage at 5 and 10°C. However, after 12 days of storage at 10°C, a reddish-brown discoloration induced by UV-C was observed on the surface of illuminated tissues, which suggests the accumulation of phenolic compounds.

No effects were reported by Allende and Artés (2003a) on MP Red Oak Leaf lettuce treated with up to 8.14 kJ/m2 UV-C on sensory quality evaluated in terms of overall visual quality, colour and browning, during storage in MAP at 5°C for 8 days. On the other hand, for MP Lollo Rosso lettuce stored at 5°C for 8 days, Allende and Artés (2003b) reported a beneficial effect of UV-C. No significant differences were found for aroma, texture, taste and colour, but samples treated with the highest fluences (2.44–8.14 kJ/m2) had better-preserved overall visual quality and presented less browning than untreated samples or samples treated with the lowest fluences (0.407 and 0.814 kJ/m2), and a shelf-life prolongation may be estimated based on sensory scores. Lettuce tissue became shinier when the highest fluence was applied, which was attributed to a possible induction of lignification-like processes started by the lettuce tissue to protect itself against the UV-C stress.

Allende et al. (2006) illuminated two sides of MP Red Oak Leaf lettuce and reported no effect on overall visual quality of UV-C illumination at fluences up to 2.37 kJ/m2, but 7.11 kJ/m2 induced tissue softening and browning after 7 days of storage at 5°C. Polyphenol oxidase is the enzyme responsible for enzymatic browning of many types of produce. Manzocco et al. (2009) achieved complete inactivation of mushroom polyphenol oxidase in vitro by UV-C illumination.

1.2.5 Effects on the Nutritional and Phytochemical Composition of MPV

It is very well known by post-harvest experts that UV illumination acts as an elicitor of resistance mechanisms in fruit and vegetables, and thus leads to a rapid increase of stress-response compounds such as phenols, flavonoids and phytoalexins, which have phytochemical properties. This change can be instantaneous or progressive and observed during produce storage. The biosynthesis of phenolic compounds is affected by UV illumination due to the increased activity of phenylalanine ammonia-lyase (Schreiner and Huyskens-Keil, 2006). Among the different controlled abiotic stresses useful to enhance the nutraceutical content of MPV, UV illumination is considered to be of high potential by the industry (Cisneros-Zevallos, 2003). There are no studies yet on the effect of UV-C on phytochemical composition of MPV. Nevertheless, increasing evidence of positive effects on whole produce and fresh-cut fruits suggests positive results, such as that reported by Cantos et al. (2001). Trans-resveratrol is a phytoalexin with health-promoting properties. Those authors demonstrated that UV-C can increase the concentration of phytoalexins in table grapes as much as 10-fold during storage. UV-C illuminated whole peppers (Vicente et al., 2005) and broccoli heads (Costa et al., 2006) showed increased antioxidant capacity with respect to controls during storage. Similarly, UV-C illuminated fresh-cut mango (González-Aguilar et al., 2007) banana and guava, but not pineapple (Alothman et al., 2009), showed increased antioxidant capacity but also vitamin C degradation.

1.3 Pulsed Light

1.3.1 Definition

Pulsed light (PL) is a new method intended for decontamination of food surfaces by killing microorganisms using short-term, high-frequency pulses of an intense broad-spectrum illumination rich in UV-C light (Gómez-López et al., 2005a). It derives from CW UV, with the main difference that PL delivers much higher fluence rates and uses xenon flash lamps instead of mercury lamps, and special hardware to produce a high peak power. Peak power is defined as the pulse energy divided by the pulse duration (Rice and Ewell, 2001). Very comprehensive reviews on the subject have been published by Elmnasser et al. (2007) and Gómez-López et al. (2007a); this section is an update, with a focus on MPV. It is common in the literature to find reports comparing the efficiency of PL versus low-pressure or medium-pressure UV lamps, where the biggest difficulty is comparing light sources which differ not only in peak power but also in emission spectrum. Recently, Bohrerova et al. (2008) compared the disinfection efficiency of PL and CW UV from low- and medium-pressure mercury lamps over Escherichia coli cells and phages T3 and T4, which were all inactivated more efficiently by PL at equivalent fluence levels.

PL has similar advantages and disadvantages to CW UV-C, but also some specific pros and cons. Perhaps the most important advantage of PL is the fast microbial inactivation. Specific disadvantages are high cost and that this process can increase produce temperature. When studying the inactivation of Aspergillus niger spores on corn meal, Jun et al. (2003) found that some experimental factor settings resulted in sample temperatures of 120°C.

1.3.2 Inactivation Mechanism

Xenon flash lamps have an emission spectrum ranging from ultraviolet to infrared light, wherein the UV-C component is the most important for microbial inactivation. Rowan et al. (1999) reported that using a flash with high UV content the inactivation of seven microorganisms was 5–6 log colony-forming units (CFU)/plate, but only 1–2 log CFU/plate with a low-UV flash. The germicidal efficiency of xenon flash lamps determined against E. coli was found to be maximum around 270 nm, with a major contribution to inactivation in the 220–290 nm range (Wang et al., 2005). Other wavelengths also contribute to microbial inactivation. Bohrerova et al. (2008) reported that a significant fraction of the enhanced PL inactivation efficiency compared to low- and medium-pressure mercury lamps was due to wavelengths greater than 295 nm, and even due to visible light in the case of viruses.

Different kinds of damage have been reported on viruses and microbial cells due to PL action. Lethal events are photochemical and/or photothermal. The primary lethal effect of PL, as with CW UV light, is the formation of cyclobutane pyrimidine dimers, as observed in cells of Saccharomyces cerevisiae (Takeshita et al., 2003) and E. coli (Bohrerova et al., 2008). Interestingly, the higher number of thymine dimers observed in flashed E. coli cells in comparison with CW UV-treated cells was attributed to an effect of light higher than 295 nm. Single-strand DNA breaks and cell-membrane damage have been reported for yeasts by Takeshita et al. (2003).

Cell rupture at fluences exceeding 0.5 J/cm2 due to momentary overheating caused by absorbing all UV light from a flash lamp was revealed by Wekhof et al. (2001). Micrographs of flashed A. niger spores were presented showing severe deformation and rupture attributed to escape of the overheated contents of the spore. Bohrerova et al. (2008) have suggested that besides DNA damage, phage capsids might be ruptured by the visible portion of PL. As in CW UV treatments, photoreactivation can also occur after PL treatment (Otaki et al., 2003).

In vitro studies performed by Gómez-López et al. (2005b) showed that proteins and oils decreased the decontamination effect of PL, while carbohydrates and water showed variable effects. For this reason MPV seem to be a suitable matrix for efficient PL decontamination.

1.3.3 Effect on Microbial Populations

PL has been proved to be effective against viruses (Roberts and Hope, 2003), Gram-negative bacteria (Sharma and Demirci, 2003), Gram-positive bacteria (Krishnamurthy et al., 2004), bacterial spores (McDonald et al., 2002), yeasts (Takeshita et al., 2003), conidia (Marquenie et al., 2003), fungal spores (Jun et al., 2003) and parasites (Huffman et al., 2000).

Research on the inactivation of human pathogens on vegetable surfaces is still pending. Regarding microorganisms naturally present on vegetables, Hoornstra et al. (2002) were first in demonstrating the potential of PL. The authors treated white cabbage, leek, paprika, carrots and kale with two pulses of PL amounting to 0.30 J/cm2. The reduction in aerobic count at the surface of the vegetables varied from 1.6 log CFU/cm2 for carrots to more than 2.6 log CFU/cm2 for paprika. Gómez-López et al. (2005b) reported a reduction in mesophilic aerobic counts between 0.56 and 2.04 log CFU/g for spinach, radicchio, iceberg lettuce, white cabbage, carrots, green bell pepper and soybean sprouts. Later on, Kaack and Lyager (2007) used PL to inactivate S. cerevisiae cells inoculated onto carrot pieces.

In order to prove whether microbial inactivation brings about shelf-life extension of MPV, Gómez-López et al. (2005b) flashed shredded iceberg lettuce and shredded white cabbage, and stored them under modified-atmosphere packaging at 7°C. For iceberg lettuce, even though a 0.46 log reduction was achieved in psychrotroph counts, control and flashed samples did not last 3 days of storage due to excessively high psychrotroph counts and bad sensory quality. For white cabbage, a 0.54 log decrease in psychrotroph counts was achieved, but after 2 days of storage control and flashed samples had similar psychrotroph counts. It is worth noting that this kind of results is common for MPV shelf-life studies, and might be avoided using storage temperatures not higher than 4°C (Gómez-López et al., 2008a).

1.3.4 Effect on Sensory Attributes

Little is known on the effect of PL on MPV. Except for some discoloration of iceberg lettuce, no adverse effects of PL on sensorial quality were reported by Hoornstra et al. (2002) after 7 days of storage at 7 or 20°C of five illuminated vegetables. Heating was a concern for Gómez-López et al. (2005b), who were unable to efficiently treat grated carrots without avoiding its dehydration. The same authors reported the appearance of off-odour in MP cabbage immediately after treatment, which disappeared overnight. MP cabbage stored at 7°C was sensorially acceptable for up to 9 days, while untreated controls were rejected after 7 days, which means a shelf-life extension of 2 days from the sensorial point of view. For MP lettuce, however, no shelf-life extension was achieved by PL treatment.

1.3.5 Effects on the Nutritional and Phytochemical Composition of MPV

There is a lack of studies about the nutritional and phytochemical consequences of the application of PL to fruit and vegetables, but encouraging results for phytochemical synthesis enhancement by CW UV makes PL a potential fast method for phytochemical content improvement. Additionally, PL did not affect levels of riboflavin and vitamin C in beef, chicken and fish (Dunn et al., 1995).

1.4 Electrolysed Oxidizing Water

1.4.1 Definition

Electrolysed oxidizing water (EOW) is created by electrolysis of diluted sodium chloride solutions in an electrolysis chamber, having free chlorine as the major disinfection factor. The most common type of electrolytic cell is a two-cell chamber. The generation of EOW using the two-cell chamber involves reactions in cells containing positively charged and negatively charged electrodes, respectively, separated by a membrane, and through which a much diluted salt-water solution passes. By subjecting the electrodes to direct current voltage, two types of water possessing different characteristics are generated: an acidic EOW (AcEW) and an alkaline EOW (AlEW). The first one has been the most studied due to its microbicidal properties. AcEW is produced from the anode side, and is characterized by a pH of less than 3, a high oxidation-reduction potential (ORP), about 1150 mV, and the presence of HOCl. AlEW is produced from the cathode side, having a pH of 11.4 and low ORP, about −795 mV (Kim et al., 2000a).

A single stream of neutral EOW (NEW), also named mixed-oxidant solution (or MIOX), with enhanced levels of HOCl, is produced by the single-cell system, where no membrane is present. Therefore, three kinds of EOW can be produced: AcEW, AlEW and NEW (MIOX). Very comprehensive reviews on EOW has been published by Al-Haq et al. (2005) and Huang et al. (2008).

EOW can be prepared by the electrolysis of a diluted saline solution, without the use of any chemicals other than sodium chloride (Koseki et al., 2004). It has therefore less adverse impact on the environment (Kim et al., 2000a). Furthermore the raw materials, water and sodium chloride, are found virtually everywhere (Venczel et al., 1997). EOW can be generated on site (Len et al., 2000), and therefore transportation and storage of potentially hazardous chemicals are not needed (Nakagawara et al., 1998). EOW is not only a decontaminant but can also prevent enzymatic browning during storage of MPV (Koseki and Itoh, 2002).

AcEW could be more effective in inactivating microorganisms than chlorinated solutions, having the same concentration of available chlorine (Koseki et al., 2001). Consequently, there should be lower formation of chloramines and trihalomethanes. NEW has also the advantage of its neutral pH, so it does not contribute as aggressively as AcEW to the corrosion of processing equipment or irritation of hands. It is also more stable as chlorine loss is significantly reduced at pH 6–9 (Deza et al., 2003).

1.4.2 Inactivation Mechanism

It has been observed that AcEW produces blebs and breaks in the outer membrane of Pseudomonas aeruginosa, inactivates nitrate reductase and degrades chromosomal DNA (Kiura et al., 2002). There is no agreement about which is the primary factor contributing to the microbicidal activity of EOW. Factors involved are concentration of free chlorine, redox potential (ORP) and pH. Kim et al. (2000a, 2000b) concluded that ORP may be the primary factor affecting microbial inactivation of AcEW, while Nakagawara et al. (1998), Len et al. (2000), Koseki et al. (2001) and Kiura et al. (2002) have concluded that HOCl concentration is the main contributor.

Other antimicrobial compounds besides HOCl are hypothetically present in EOW, which should explain the higher effectiveness of EOW over conventional HOCl solutions to inactivate microorganisms in vitro (Venczel et al., 1997). But those compounds, such as hydroxyl radicals, have not been detected (Stan et al., 2005), and disinfection experiments on lettuce under controlled conditions of chlorine concentration, pH and ORP do not agree with this hypothesis (Park et al., 2001). Perhaps additional compounds become quickly degraded by organic matter, and are not relevant for decontamination of MPV.

Chlorine is certainly involved and pH determines the most important species: Cl2 below pH 3 (typical of AcEW), and HOCl and ClO− above pH 4 (typical of NEW) (Nakagawara et al., 1998). The maximum in vitro antibacterial activity of AcEW occurs around pH 4 (Nakagawara et al., 1998; Len et al., 2000; Park et al., 2004). However, results from in vivo tests do not confirm the applicability of in vitro results. Yang et al. (2003) dipped MP lettuce in EOW at different pHs, and found no pH effect for the inactivation of Salmonella Typhimurium and Listeria monocytogenes, and two inactivation peaks for E. coli O157:H7 at pH 4 and 8.

1.4.3 Effect on Microbial Populations

Different studies have proved the efficacy of EOW to inactivate human pathogens both in vitro (Venczel et al., 1997; Venkitanarayanan et al.1999; Kim et al., 2000a, 2000b; Nakajima et al., 2004; Park et al., 2004) and inoculated onto vegetable surfaces (see Table 1.1) (Deza et al., 2003; Abadías et al., 2008; Park et al., 2008).

Table 1.1 Studies on the effect of electrolysed water on pathogenic microorganisms inoculated onto fresh or minimally processed vegetables.

As for spoilage microorganisms, Izumi (1999) studied the effect of rinsing five MPV with NEW (20 ppm available chlorine, pH 6.8, 4 min) on mesophilic aerobic microorganisms, finding a 1.8 log reduction for trimmed spinach leaves to no significant effect for carrot slices, Japanese radish shreds or diced potatoes. The author concluded that the effect of NEW was influenced by the surface area, anatomy and microstructure of the tissues, which differ among vegetables, as well as the type of cut, which would affect the extent of contact of EOW with microorganisms. Koide et al. (2009) reported 1.5 and 1.3 log reductions in total aerobic bacteria, yeast and mould counts after dipping MP cabbage in NEW (20 mg/l, 10 min). As for shelf-life prolongation, Rico et al. (2008) achieved 1.4–1.6 log reduction in aerobic mesophilic counts on lettuce compared to water washing, a difference that persisted after 7 days of storage at 4°C.

Regarding AcEW, it reduced total aerobic plate count of MP cilantro leaves by 0.66 log compared to water washing (Wang et al., 2004), by more than 1 log in whole chinjon, leafy cabbage, spinach, cucumber and snap beans, and just 0.9 log in green peppers. With AlEW alone, a no more than 0.5 log reduction was observed (Lin et al., 2005). Monitoring the effect of AcEW during the storage of MP cilantro at 0°C, Wang et al. (2004) observed that the total aerobic plate count and total Enterobacteriaceae count exhibited a 4-day lag phase. AcEW (50 ppm free chlorine, pH 2.8) reduced E. coli O157:H7 counts by 0.72 and 0.77 logarithmic cycles in MP iceberg and Romaine lettuce respectively (Keskinen et al., 2009).

AlEW lacks antimicrobial properties, but it is considered to act like a diluted sodium hydroxide solution. Thus, it would work like a surface-active agent on vegetable surfaces. Consequently, the microorganisms on the surface would be reached more easily by AcEW during a sequential process, which explains higher decontamination found in lettuce (Koseki et al., 2001), whole cucumber (Koseki et al., 2004) and MP lettuce and MP cabbage (Koseki and Itoh, 2001, 2002). The shelf life of the latter MPV was estimated to be extended by at least 1 day in spite of bacteria growing faster in samples treated with AcEW than in water-washed samples.

1.4.4 Effect on Sensory Quality

EOW washing does not change sensory properties of vegetables immediately after treatment. It does not affect the surface colour of carrot slices, trimmed spinach leaves or cucumber slices (Izumi, 1999); the taste, appearance or smell of whole tomatoes (Deza et al., 2003); or the appearance of whole cucumbers (Lin et al., 2005), MP lettuce, carrot and endive (Abadías et al., 2008). Panellists of triangle tests were unable to differentiate MP iceberg lettuce, white cabbage and carrots treated with NEW (40 mg/l free chlorine, 5 min) from water-washed samples (Gómez-López et al., 2008b). A residual chlorine odour after washing vegetables with AcEW (50 ppm active chlorine) can be eliminated by soaking with AlEW (Lin et al., 2005). Furthermore, Park et al. (2001) found no effects of AcEW on visual quality, stem discoloration, wilting and colour of whole lettuce leaves stored for 14 days at 4°C.

As for shelf-life prolongation, Gómez-López et al. (2007b) extended the shelf life of shredded cabbage stored at 4°C by 5 days due to treatment with NEW. Rico et al. (2008) also showed beneficial effects of increasing concentrations of free chlorine (12–120 ppm) in NEW to decrease browning potential of lettuce and polyphenol oxidase activity and improving sensory quality evaluated in terms of fresh appearance, photosynthetic and vascular browning, and general acceptability; although high free-chlorine content NEW produced changes associated to blanching and loss of crispness.

AcEW can have beneficial effects in the sensorial stability of some MPV during refrigerated storage, such as decreasing the progress of browning in MP lettuce and MP cabbage (Koseki and Itoh, 2002). The authors suggested the enzymes responsible for browning may have been oxidized and weakened by the strong ORP of AcEW. But AcEW can also have deleterious effects, such as loss of aroma of cilantro leaves during storage, possibly correlated, according to the authors, to a higher tissue electrolyte leakage caused by oxidation of the cilantro cell membrane by AcEW (Wang et al., 2004).

1.4.5 Effects on the Nutritional and Phytochemical Composition of MPV

Treating MP leek with NEW up to 30 mg/l free chlorine compared to water-wash treatment did not influence vitamin C, α-tocopherol and total phenol content, the antioxidant capacity measured by the ferric reducing antioxidant power (FRAP) method, and the concentration of the carotenoids all-trans-β-carotene and 9-cis-β-carotene; but decreased violaxanthin and lutein concentrations, as well as γ-tocopherol content (Vandekinderen et al., 2009).

1.5 Ozone

1.5.1 Definition

Ozone (O3) is a highly unstable triatomic molecule that is formed by addition of an oxygen atom to molecular diatomic oxygen. When a free oxygen atom encounters molecular oxygen, it combines to form the ozone molecule. Production of ozone is generally accomplished by using the corona discharge method (Güzel-Seydim et al., 2004). Ozone is a strong oxidant active against all types of microorganism. Excess ozone auto-decomposes rapidly to produce oxygen, and thus it leaves no residues in food. It can be applied as a gas, or in aqueous solution where the gas does not appreciably react with water; therefore it forms a true physical solution (Khadre et al., 2001).

The use of this disinfectant has some limitations. Because of its instability, ozone must be generated at the usage site. Metal and other types of surfaces with which it comes into contact are subject to corrosion or other deterioration because of its strong oxidizing power (Beuchat, 1998). Several food components interfere with its microbicidal efficacy (Restaino et al., 1995; Güzel-Seydim et al., 2004). Decomposition of ozone is so rapid in the water phase of foods that its antimicrobial activity is restricted to surfaces; therefore microorganisms embedded in product surfaces are more resistant to ozone than those readily exposed to sanitizer (Mahapatra et al., 2005). Ozone-detection and -destruction systems and respirators are needed for the safety of workers in food-processing facilities (Kim et al., 1999b).

1.5.2 Inactivation Mechanism

Ozone is a strong oxidant and disinfectant. It can react with contaminants directly as molecular ozone or indirectly as ozone-derived free radicals such as OH and H2O (Koseki and Itoh, 2001). Ozone decomposes in solution in a stepwise fashion, producing in turn hydroperoxyl (HO2), hydroxyl (OH) and superoxide (O2−) radicals. The hydroxyl radical is an important transient species and chain-propagating radical. The reactivity of ozone is attributed to the great oxidizing power of these free radicals (Kim et al., 1999b). Different targets have been proposed for its bactericidal action: double bonds of unsaturated lipids in the cell envelope, interference with the respiratory system and damage to the genetic material, and a general oxidation of protoplasm (Kim et al., 1999b). Young and Setlow (2004) proposed that spore killing by ozone is due to some type of damage to the spore's inner membrane, although the identity of this damage was not clear.

1.5.3 Ozonated Water

1.5.3.1 Effect on Microbial Populations

The effect of ozonated water on pathogen populations of vegetable surfaces is very variable. Singh et al. (2002) reported 1.42 and 1.80 log reductions respectively in E. coli O157:H7 after treating MP lettuce and baby carrots with ozonated water (16.5 mg/l, 10 min). In contrast, counts of L. monocytogenes and E. coli O157:H7 in shredded lettuce were reduced from 6.0 log CFU/g to less than 1.0 log CFU/g by washing with ozonated water (3 ppm, 5 min) (Rodgers et al., 2004). Finally, counts of Shigella sonnei inoculated on shredded lettuce decreased 1.7 log units by ozonated water (5 ppm, 5 min) (Selma et al., 2007); and counts of L. monocytogenes in green leaf lettuce were reduced by more than 2 log units (Ölmez and Akbas, 2009).

Ozonated water has also shown mixed effectiveness to reduce microorganisms naturally present in MPV. Kim et al. (1999a) observed that bubbling ozone while stirring at high velocity (300 rpm) was the best of different alternatives tested to decontaminate lettuce, with log reductions of 1.9 log CFU/g for both counts of mesophilic and psychrotrophic bacteria. Thereafter, a number of articles have reported from non-significant effects to almost 2-log reductions (Table 1.2).

Table 1.2 Studies on the effect of ozonated water on natural microflora of fresh or minimally processed vegetables.

MPV decontaminated by ozonated water and stored at 4°C have been shown to have longer shelf life than their untreated counterparts, from the microbiological point of view. Counts of mesophilic bacteria in ozonated MPV remained lower than in controls in the cases of shredded lettuce for up to 9 days (Rodgers et al., 2004) and 13 days (Beltrán et al., 2005b); and fresh-cut celery for up to 9 days (Zhang et al., 2005). For ozonated MP potato, mesophilic bacteria counts were not different from controls during storage; however, no decontamination was observed due to treatment (Beltrán et al., 2005a).

1.5.3.2 Effect on Sensory Attributes

It has been shown that ozonated water maintains the sensory quality of MPV, among which MP lettuce is the most studied. According to microscopy observations, lettuce surface is not affected by ozone (Koseki et al., 2001). Ozonated water had positive effects on sensory quality of MP lettuce stored at 4°C for 9 days (Ölmez and Akbas, 2009), the shelf life of which can be 4 days longer than chlorinated samples (Garcia et al., 2003). Samples of MP lettuce washed with ozonated water and stored in air maintained an excellent visual quality during storage at 4°C up to 13 days without significant differences compared to the initial visual quality, and no browning was observed. In contrast, water-washed controls decreased in visual quality and browned sharply after 5 days. Moreover, ozone did not affect its texture (Beltrán et al., 2005b).

Fresh-cut cilantro leaves washed with ozonated water and stored at 0°C for up to 14 days had the same colour as leaves washed with tap water. The typical cilantro aroma was maintained with a higher score by the ozone treatment at day 14. The ozonated samples exhibited better overall quality retention during storage. The leaves appeared to be near the fresh or initial conditions of the cilantro, with a green and fresh appearance, no yellowing or dehydration and no trace of off-odour (Wang et al., 2004). Ozonated water (0.18 ppm) was beneficial in maintaining colour, visible structural integrity and general appearance of MP celery during storage at 4°C (Zhang et al., 2005). As for MP rocket leaves, ozone (10 mg/l) affected neither colour nor visual quality (Martínez-Sánchez et al., 2006).

Potato strips treated with ozonated water (20 mg/l) stored under vacuum at 4°C during 14 days showed no evidence of browning, and maintained the full typical aroma and a very firm and turgid texture (Beltrán et al., 2005a). Treatment of MP green asparagus with 1 mg/l aqueous ozone for 30 min delays the synthesis of the structural cell-wall constituents that lead to asparagus-toughening lignin, cellulose and hemicelluse during storage at 3°C for 25 days (An et al., 2007).

Shredded carrots were affected by immersion in ozonated water (1 ppm, 5 min), suffering from loss of colour due to leaching, softening, losses of perceived aroma and low general acceptance, although the highly exposed cut area could account for the observed losses (Alegria et al., 2009).

1.5.4 Gaseous Ozone

1.5.4.1 Factors Affecting the Efficacy of Ozone Gas

The factors affecting the decontaminant efficacy of ozone gas were studied by Han et al. (2002) in E. coli O157:H7 inoculated onto green bell pepper surface. The order of significance and from the most important to the least was: ozone concentration (2–8 mg/l), exposure time (10–40 min) and relative humidity (60–90%), in the ranges indicated. A synergism was found between gas concentration and relative humidity. Li and Wang (2003) also found that germicidal efficiencies over four microorganisms increased as relative humidity increased, which could be related to the higher number of radicals from the ozone reaction with more water vapour at higher relative humidity.

1.5.4.2 Effect on Pathogenic Microorganisms and Sensory Quality

There are few studies about the application of ozone in the gas phase to decontaminate MPV. Singh et al. (2002) reported 1.79 and 2.64 log reductions in E. coli O157:H7 populations after treating respectively MP lettuce and baby carrots with gaseous ozone (7.6 mg/l, 15 min). Decoloration of lettuce leaves was observed after treatment with 5.2 or 7.6 mg/l O3 for 10 and 15 min. Han et al. (2002) reported 7.35 log reduction (log CFU/5 g) of E. coli O157:H7 inoculated onto green bell pepper surface after treatment with 8 mg/l O3 at 90% relative humidity for 25 min. Klockow and Keener (2009) developed an ozone-generation system where bags of vegetables, spinach in this case, were subjected to an electric field to generate ozone, and then stored. E. coli O157:H7 populations decreased by 5.8 and more than 6.4 log CFU/leaf after 24 h of storage at 5 or 22°C respectively, but leaf colour changed from green to white, especially at the stem and outer edges of the leaf.

1.5.5 Effects on the Nutritional and Phytochemical Composition of MPV

As with other disinfectants, the effect of ozonated water has been primarily studied in MP iceberg lettuce, while the effect of gaseous ozone is still pending for tests. It seems that in general ozone has neutral nutritional consequences for MPV. Ozonated water at 5 ppm, 5 min (Koseki and Isobe, 2006) and at 2–4 ppm, 2 min (Akbas and Ölmez, 2007; Ölmez and Akbas, 2009) did not affect the ascorbic acid content of MP lettuce; although harder treatments (10 or 20 mg/l O3) did reduce vitamin C content (Beltrán et al., 2005b). In contrast, 0.03 and 0.08 ppm of ozone in washing water delayed the oxidation of vitamin C in MP celery during storage at 4°C up to 9 days (Zhang et al., 2005). According to the authors, inhibition of polyphenol oxidase and delay of tissue metabolism by ozonated water could account for this effect.

Ozone (10 mg/l) had no effect on the total polyphenol content of MP lettuce (Beltrán et al., 2005b) or rocket leaves (Martínez-Sánchez et al., 2006), and ozonated water (2–4 ppm, 2 min) did not affect β-carotene content of MP lettuce (Akbas and Ölmez, 2007; Ölmez and Akbas, 2009).

1.6 Low-Temperature Blanching

1.6.1 Definition

Low-temperature blanching consists of immersion of MPV in hot water for up to 50°C and 120 s, high enough to provoke beneficial physiological changes and microbial reduction without cooking. Most of the literature on low-temperature blanching has been published in articles dealing with the effect of warm chlorinated water. However, because of the concerns on the use of chlorine for decontamination of fruits and vegetables, only the results of blanching in pure water will be summarized in this section.

1.6.2 Effect on Microbial Populations

The first results on the effect of mild heat treatment on the stability of MPV were discouraging from the microbiological point of view. Compared to samples washed at 20°C, immersion of MP lettuce in warm water (50°C, 90 s) decreased psychrotrophic counts by 0.56 log, but did not significantly affect counts of mesophilic microorganisms, Enterobacteriaceae, and yeasts and moulds. After less than 4 days of storage at 5 or 15°C the effect on psychrotroph counts was lost (Li et al., 2001). Experiments under identical conditions showed that mild heat treatment enhances growth of L. monocytogenes during subsequent storage (Li et al., 2002). The enhanced growth of microorganisms after a decontamination process is also frequent for other decontamination techniques as revised by Gómez-López et al. (2008a).

Compared to washing at 20°C for respective times, washing lettuce with warm water (50°C, 1 or 5 min) reduced counts of E. coli O157:H7 by 1.68 and 1.98 logs respectively, and Salmonella counts by 1.51 and 2.03 logs (Koseki et al., 2004). A more than 1.0 log reduction was reported for total bacteria, pseudomonades and Enterobacteriaceae after pre-washing of iceberg lettuce with warm water (50°C, 60 s) after trimming and coring but before shredding in comparison with washing with cold water (4°C, 90 s) after shredding. The sanitizing effect persisted throughout storage (4°C, 9 days) (Baur et al., 2005). Similarly results were reported by Klaiber et al. (2005) for aerobic mesophilic bacteria, lactic acid bacteria and enterobacteria of shredded carrots pre-washed in warm water (50°C, 120 s) after peeling and topping but before shredding, in comparison with non-washed samples.

1.6.3 Effects on Sensory Quality

The main beneficial effect of low-temperature blanching is suppression of lettuce browning. Immersion in warm water (50°C, 90 s) retarded browning for at least 7 days at 5°C, and 2 days at 15°C (Li et al., 2001). Fukumoto et al. (2002) studied the effect of warm water (47°C, 3 min) compared with cold water (4°C) on the photosynthetic and vascular tissues of iceberg lettuce stored for 7 days at 5°C. Washing at 47°C strongly inhibited edge browning, phenylalanine ammonia-lyase activity and peroxidase activity. Baur et al. (2005) reported that pre-washing of MP iceberg lettuce with warm water (50°C, 60 s) decreased cut-edge vascular tissue browning and phenylalanine ammonia-lyase during storage (4°C, 9 days).

Low-temperature blanching has been also applied to other MPV. Colour and odour of low temperature blanched MP endives stored for 2 days at 4°C were negatively affected when blanching conditions were 50°C/10 min. When 52.5°C/10 min and 55°C/5 min conditions were used, appearance, taste and texture were additionally damaged (Mayer-Miebach et al., 2003). Pre-washing of MP carrots with warm water (50°C, 120 s) did not affect overall visual quality, colour, odour, texture, sweetness and flavour during storage (4°C, 9 days) as determined by sensory panel; it also did not affect whiteness index, and contents of glucose, fructose, sucrose, as well as total sugar. However, longer blanching times (60°C, 40 min) decreased carrot hardness due to pectin demethoxylation (Lemmens et al., 2009).

1.6.4 Effects on the Nutritional and Phytochemical Composition of MPV

Low-temperature blanching of detached whole endive leaves at conditions 50°C/10 min, 52.5°C/10 min and 55°C/5 min reduced polyphenol contents and antioxidant capacity (Mayer-Miebach et al., 2003). In contrast, blanching carrots at 60°C for 40 min did not modify β-carotene levels of carrots (Lemmens et al., 2009).

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