QUANTITATIVE ESTIMATIONS OF URINE & BLOOD CONSTITUENTS - vishalbhai chudasama - E-Book

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Beschreibung

I kirankumar suthar write book of biochemistry and clinical pathology practical topic. Which is useful for student of pharmacy,Nursing and medical proffession.

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Veröffentlichungsjahr: 2015

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kirankumar suthar, bharatbhai rajpurohit

QUANTITATIVE ESTIMATIONS OF URINE & BLOOD CONSTITUENTS

BookRix GmbH & Co. KG81371 Munich

INDEX

INDEX

QUANTITATIVE ESTIMATIONS OF URINE & BLOOD CONSTITUENTS

Sr.No.

Aim of experiment

Page.No.

1.

Introduction to the Laboratory, subject.

 

 

2

2.

Estimation of Chlorides in Urine (Whitehorn's Method).

 

 

6

3.

To determine titrable acidity and ammonia in urine.

 

 

9

 

 

4.

To determine the glucose content in urine by Benedict’s method.

 

 

12

 

 

5.

To determine the glucose content in urine by Cole’s ferricyanide – Methylene blue method.

 

 

15

 

6.

Estimation of serum calcium.

 

 

18

 

7.

Introduction to colorimetric analysis.

 

 

21

 

8.

To estimate total proteins in plasma by biuret method.

 

 

23

 

9.

To estimate creatinine in blood.

 

 

26

 

10.

To estimate total cholesterol in plasma.

 

 

29

 

11.

References

 

 

32

 

Introduction to the Laboratory, subject, list of requirement.

Experiment No: 1                                                                          

 

 

 

Aim: Introduction to the Laboratory, subject, list of requirement.

 

Introduction to the Laboratory:

 

Laboratory work has special importance in the learning of science as scientific principles develop and grow on the basis of experimental work. Chemistry is an experimental science.

 

Laboratory work provides an opportunity to observe many of the chemical phenomena under controlled laboratory conditions. Hence, it provides you with ample opportunity to observer and to draw inferences and explain results.

 

Before you start working in the laboratory, you should be well acquainted with the biochemistry laboratory. You should notice the facilities provided in the laboratory and on your working table.

 

You will notice that your working table is provided with a sink, water-tap, gas-tap, Bunsen burner with guard cum stand, a reagent shelf and a bin for disposing waste material. You will find that some reagents are kept on the shelf fixed on the table while some reagents are kept on the shelf fixed on sidewalls. Reagents kept on the shelf of the table are frequently required while those on the shelf fixed on walls are less commonly used.