Photonics, Volume 4 E-Book

David L. Andrews

• Herausgeber: John Wiley & Sons
• Kategorie: Fachliteratur
• Serie: A Wiley-Science Wise Co-Publication
• Sprache: Englisch

Seitenzahl: 1202

Veröffentlichungsjahr: 2015

Beschreibung

Discusses the basic physical principles underlying Biomedical Photonics, spectroscopy and microscopy

This volume discusses biomedical photonics, spectroscopy and microscopy, the basic physical principles underlying the technology and its applications. The topics discussed in this volume are: Biophotonics; Fluorescence and Phosphorescence; Medical Photonics; Microscopy; Nonlinear Optics; Ophthalmic Technology; Optical Tomography; Optofluidics; Photodynamic Therapy; Image Processing; Imaging Systems; Sensors; Single Molecule Detection; Futurology in Photonics.

• Comprehensive and accessible coverage of the whole of modern photonics
• Emphasizes processes and applications that specifically exploit photon attributes of light
• Deals with the rapidly advancing area of modern optics
• Chapters are written by top scientists in their field

Written for the graduate level student in physical sciences; Industrial and academic researchers in photonics, graduate students in the area; College lecturers, educators, policymakers, consultants, Scientific and technical libraries, government laboratories, NIH.

Leseprobe

PHOTONICS

Scientific Foundations, Technology and Applications

Biomedical Photonics, Spectroscopy, and Microscopy Volume IV

Edited by

David L. Andrews

School of Chemical Sciences University of East Anglia Norwich, UK

Copyright © 2015 by John Wiley & Sons, Inc. All rights reserved.

Published by John Wiley & Sons, Inc., Hoboken, New Jersey. Published simultaneously in Canada.

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CONTENTS

List of Contributors

Preface

Chapter 1: Fluorescence

1.1 Introduction

1.2 Spectra

1.3 Quantum Yield

1.4 Lifetime

1.5 Quenching

1.6 Anisotropy

1.7 Microscopy

1.8 Conclusions

Acknowledgments

Notes

References

Chapter 2: Single-Molecule Detection and Spectroscopy

2.1 Introduction

2.2 Experimental Setups

2.3 Fluorescence Spectroscopy

2.4 Fluorescence Correlation Spectroscopy

2.5 Fluorescence Excitation Spectroscopy

2.6 Other Detection Methods

2.7 Conclusion

Acknowledgments

References

Chapter 3: Resonance Energy Transfer

3.1 Introduction

3.2 History of RET

3.3 The Photophysics of RET

3.4 Investigative Applications of RET in Molecular Biology

3.5 The Role of RET in Light-Harvesting Complexes

Acknowledgments

References

Chapter 4: Biophotonics of Photosynthesis

4.1 Introduction

4.2 Structure of Pigment–Protein Complexes and Structure–Function Relationships

4.3 Key Concepts in Physics of Pigment–Protein Complexes

4.4 Experimental Techniques

4.5 Examples

4.6 Conclusions

Acknowledgments

References

Chapter 5: Optical Sectioning Microscopy and Biological Imaging

5.1 Introduction and Background

5.2 Confocal Imaging

5.3 Nonlinear Microscopy

5.4 Practical Implementation of Nonlinear Microscopy

5.5 Recent Advances in Nonlinear Microscopy

5.6 Widefield Optical Sectioning by Specialized Illumination Methods

5.7 Summary

References

Chapter 6: Cell Handling, Sorting, and Viability

6.1 Handling Cells with Light

6.2 Optical Sorting

6.3 Cell Viability

References

Notes

Chapter 7: Tissue Polarimetry

7.1 Introduction

7.2 Polarized Light Fundamentals

7.3 Instrumentation

7.4 Forward Modeling and Testing in Phantoms

7.5 Applications

7.6 Conclusions and Outlook

References

Chapter 8: Optical Waveguide Biosensors

8.1 Introduction

8.2 Fundamentals of Label-Free Optical Waveguide Biosensing

8.3 Surface Biofunctionalization

8.4 Evaluation of Optical Biosensors

8.5 Integrated Optical Waveguide-Based Biosensors

8.6 Optical Fiber-Based Biosensors

8.7 Lab-On-A-Chip Integration

8.8 Summary

References

Chapter 9: Light Propagation in Highly Scattering Turbid Media: Concepts, Techniques, and Biomedical Applications

9.1 Introduction

9.2 Physics Behind Optical Imaging Through a Highly Scattering Turbid Medium

9.3 Study of Ballistic and Diffuse Light Components

9.4 Photon-Sorting Gates

9.5 Transition From Ballistic to Diffuse Imaging in Turbid Media

9.6 Conclusion

Acknowledgments

References

Chapter 10: Photodynamic Therapy

10.1 Historical Overview of PDT

10.2 Introduction to PDT

10.3 Photosensitizer Structure and Photophysical Properties

10.4 Light Dosimetry and Photodynamic Therapy Light Sources

10.5 Light-Based Strategies to Enhance PDT

10.6 PDT Targeting and Nanotechnology

10.7 PDT for Dermatology

10.8 PDT for Oncology

10.9 PDT for Infectious Disease

10.10 PDT in Ophthalmology

10.11 PDT and The Immune System

10.12 Conclusion

Acknowledgment

References

Chapter 11: Imaging and Probing Cells Beyond the Optical Diffraction Limit

11.1 The Quest for Achieving Optical Resolution Beyond ABBE'S Limit

11.2 Stimulated Emission Depletion Microscopy

11.3 Photoactivated Localization Microscopy and Stochastic Optical Reconstruction Microscopy

11.4 Structured Illumination Microscopy

11.5 Super-Resolution Optical Fluctuation Imaging and Other Approaches

11.6 Outlook

Acknowledgments

References

Chapter 12: Technology

12.1 Basic Ocular Anatomy and Physiology

12.2 Measurement Techniques

12.3 Anterior Segment Diagnostics, Refractive Measurements, and Treatment

12.4 Diagnostic Applications and Treatment of Posterior Segment

References

Index

EULA

List of Tables

Chapter 1

Table 1.1

Table 1.2

Table 1.3

Table 1.4

Chapter 6

Table 6.1

Table 6.2

Chapter 7

Table 7.1

Table 7.2

Table 7.3

Chapter 8

Table 8.1

Chapter 9

Table 9.1

Table 9.2

Table 9.3

Chapter 10

Table 10.1